Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis

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	Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis
	Wang, Changhe ; Wang, Yeshi ; Hu, Meiqin ; Chai, Zuying ; Wu, Qihui ; Huang, Rong ; Han, Weiping ; Zhang, Claire Xi ; Zhou, Zhuan
刊名	EMBO REPORTS
	2016
关键词	bulk endocytosis clathrin-mediated endocytosis exocytosis synaptotagmin-11 vesicle recycling SYNAPTIC-VESICLE ENDOCYTOSIS ROOT GANGLION NEURONS HIPPOCAMPAL SYNAPSES SLOW ENDOCYTOSIS NERVE-TERMINALS CA2+ BINDING DYNAMIN MEMBRANE EXOCYTOSIS MECHANISMS
英文摘要	Precise and efficient endocytosis is essential for vesicle recycling during a sustained neurotransmission. The regulation of endocytosis has been extensively studied, but inhibitors have rarely been found. Here, we show that synaptotagmin-11 (Syt11), a non-Ca(2+-)binding Syt implicated in schizophrenia and Parkinson's disease, inhibits clathrin-mediated endocytosis (CME) and bulk endocytosis in dorsal root ganglion neurons. The frequency of both types of endocytic event increases in Syt11 knockdown neurons, while the sizes of endocytosed vesicles and the kinetics of individual bulk endocytotic events remain unaffected. Specifically, clathrincoated pits and bulk endocytosis-like structures increase on the plasma membrane in Syt11-knockdown neurons. Structural-functional analysis reveals distinct domain requirements for Syt11 function in CME and bulk endocytosis. Importantly, Syt11 also inhibits endocytosis in hippocampal neurons, implying a general role of Syt11 in neurons. Taken together, we propose that Syt11 functions to ensure precision in vesicle retrieval, mainly by limiting the sites of membrane invagination at the early stage of endocytosis.; National Basic Research Program of China [2007CB512100, 2012CB518006]; National Natural Science Foundation of China [30770521, 30970660, 31171026, 31100597, 30911120491, 30830043, 31330024, 31400708, 31471085]; National Key Technology RD Program [SQ2011SF11B01041]; Beijing Natural Science Foundation Program; Scientific Research Key Program of Beijing Municipal Commission of Education [KZ201510025023]; Beijing Institute for Brain Disorders [PXM2013_014226_07_000087]; Natural Science Foundation of Heilongjiang Province of China [C201453]; A*STAR Biomedical Research Council; Peking-Tsinghua Center for Life Sciences; SCI(E); ARTICLE; clairexizhang@ccmu.edu.cn; 1; 47-63; 17
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/438941]
专题	生命科学学院心理与认知科学学院
推荐引用方式 GB/T 7714	Wang, Changhe,Wang, Yeshi,Hu, Meiqin,et al. Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis[J]. EMBO REPORTS,2016.
APA	Wang, Changhe.,Wang, Yeshi.,Hu, Meiqin.,Chai, Zuying.,Wu, Qihui.,...&Zhou, Zhuan.(2016).Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis.EMBO REPORTS.
MLA	Wang, Changhe,et al."Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis".EMBO REPORTS (2016).