Arabidopsis COP1 SUPPRESSOR 2 Represses COP1 E3 Ubiquitin Ligase   Activity through Their Coiled-Coil Domains Association

doi:10.1371/journal.pgen.1005747

CORC > 北京大学 > 生命科学学院

	Arabidopsis COP1 SUPPRESSOR 2 Represses COP1 E3 Ubiquitin Ligase Activity through Their Coiled-Coil Domains Association
	Xu, Dongqing ; Lin, Fang ; Jiang, Yan ; Ling, Junjie ; Hettiarachchi, Chamari ; Tellgren-Roth, Christian ; Holm, Magnus ; Wei, Ning ; Deng, Xing Wang
刊名	PLOS GENETICS
	2015
关键词	LIGHT-DEPENDENT INTERACTION BLUE-LIGHT TRANSCRIPTION-FACTOR TUMOR-SUPPRESSOR GENE-EXPRESSION PHOTOMORPHOGENIC DEVELOPMENT SEEDLING DEVELOPMENT PROTEIN STABILITY PLANT DEVELOPMENT CRYPTOCHROME 1
DOI	10.1371/journal.pgen.1005747
英文摘要	CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) functions as an E3 ubiquitin ligase and mediates a variety of developmental processes in Arabidopsis by targeting a number of key regulators for ubiquitination and degradation. Here, we identify a novel COP1 interacting protein, COP1 SUPPRESSOR 2 (CSU2). Loss of function mutations in CSU2 suppress the constitutive photomorphogenic phenotype of cop1-6 in darkness. CSU2 directly interacts with COP1 via their coiled-coil domains and is recruited by COP1 into nuclear speckles in living plant cells. Furthermore, CSU2 inhibits COP1 E3 ubiquitin ligase activity in vitro, and represses COP1 mediated turnover of HY5 in cell-free extracts. We propose that in csu2 cop1-6 mutants, the lack of CSU2's repression of COP1 allows the low level of COP1 to exhibit higher activity that is sufficient to prevent accumulation of HY5 in the dark, thus restoring the etiolated phenotype. In addition, CSU2 is required for primary root development under normal light growth condition.; National Natural Science Foundation [31330048]; National Basic Research Program of China (973 Program) [2012CB910900]; Peking-Tsinghua Center for Life Sciences; US NIH [GM-47850]; RFI/VR "NGI" National Genomics Infrastructure and Science for Life Laboratory, Uppsala; SCI(E); PubMed; ARTICLE; ning.wei@yale.edu; deng@pku.edu.cn; 12; e1005747; 11
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/435595]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, Dongqing,Lin, Fang,Jiang, Yan,et al. Arabidopsis COP1 SUPPRESSOR 2 Represses COP1 E3 Ubiquitin Ligase Activity through Their Coiled-Coil Domains Association[J]. PLOS GENETICS,2015.
APA	Xu, Dongqing.,Lin, Fang.,Jiang, Yan.,Ling, Junjie.,Hettiarachchi, Chamari.,...&Deng, Xing Wang.(2015).Arabidopsis COP1 SUPPRESSOR 2 Represses COP1 E3 Ubiquitin Ligase Activity through Their Coiled-Coil Domains Association.PLOS GENETICS.
MLA	Xu, Dongqing,et al."Arabidopsis COP1 SUPPRESSOR 2 Represses COP1 E3 Ubiquitin Ligase Activity through Their Coiled-Coil Domains Association".PLOS GENETICS (2015).