RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating   Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis

doi:10.1105/tpc.15.00949

CORC > 北京大学 > 生命科学学院

	RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis
	Wu, Zhe ; Zhu, Danling ; Lin, Xiaoya ; Miao, Jin ; Gu, Lianfeng ; Deng, Xian ; Yang, Qian ; Sun, Kangtai ; Zhu, Danmeng ; Cao, Xiaofeng ; Tsuge, Tomohiko ; Dean, Caroline ; Aoyama, Takashi ; Gu, Hongya ; Qu, Li-Jia
刊名	PLANT CELL
	2016
关键词	SERINE/ARGININE-RICH PROTEIN COLD ADAPTATION PROCESS TRANSCRIPTION FACTOR SHOOT MERISTEM POLYMERASE-II SR PROTEINS NASCENT RNA THALIANA REVEALS SEQ
DOI	10.1105/tpc.15.00949
英文摘要	Nuclear-localized RNA binding proteins are involved in various aspects of RNA metabolism, which in turn modulates gene expression. However, the functions of nuclear-localized RNA binding proteins in plants are poorly understood. Here, we report the functions of two proteins containing RNA recognition motifs, RZ-1B and RZ-1C, in Arabidopsis thaliana. RZ-1B and RZ-1C were localized to nuclear speckles and interacted with a spectrum of serine/arginine-rich (SR) proteins through their C termini. RZ-1C preferentially bound to purine-rich RNA sequences in vitro through its N-terminal RNA recognition motif. Disrupting the RNA binding activity of RZ-1C with SR proteins through overexpression of the C terminus of RZ-1C conferred defective phenotypes similar to those observed in rz-1b rz-1c double mutants, including delayed seed germination, reduced stature, and serrated leaves. Loss of function of RZ-1B and RZ-1C was accompanied by defective splicing of many genes and global perturbation of gene expression. In addition, we found that RZ-1C directly targeted FLOWERING LOCUS C (FLC), promoting efficient splicing of FLC introns and likely also repressing FLC transcription. Our findings highlight the critical role of RZ-1B/1C in regulating RNA splicing, gene expression, and many key aspects of plant development via interaction with proteins including SR proteins.; National Natural Science Foundation of China [31230006, 31211140041]; 111 Project; SCI(E); PubMed; ARTICLE; qulj@pku.edu.cn; 1; 55-73; 28
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/435550]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wu, Zhe,Zhu, Danling,Lin, Xiaoya,et al. RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis[J]. PLANT CELL,2016.
APA	Wu, Zhe.,Zhu, Danling.,Lin, Xiaoya.,Miao, Jin.,Gu, Lianfeng.,...&Qu, Li-Jia.(2016).RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis.PLANT CELL.
MLA	Wu, Zhe,et al."RNA Binding Proteins RZ-1B and RZ-1C Play Critical Roles in Regulating Pre-mRNA Splicing and Gene Expression during Development in Arabidopsis".PLANT CELL (2016).