Fertilization Independent Endosperm genes repress NbGH3.6 and regulate   the auxin level during shoot development in Nicotiana benthamiana

doi:10.1093/jxb/erw024

CORC > 北京大学 > 生命科学学院

	Fertilization Independent Endosperm genes repress NbGH3.6 and regulate the auxin level during shoot development in Nicotiana benthamiana
	Zeng, Jun ; Ding, Qi ; Fukuda, Hiroo ; He, Xin-Qiang
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2016
关键词	Auxin FIE GH3.6 Nicotiana benthamiana shoot development STEM-CELL NICHE POLYCOMB REPRESSION HORMONAL-REGULATION PLANT ARCHITECTURE ARABIDOPSIS EXPRESSION VIRUS FIE MAINTENANCE CYTOKININ
DOI	10.1093/jxb/erw024
英文摘要	The Fertilization Independent Endosperm (FIE) gene is required to restrict endosperm development without fertilization, and it represses flowering during embryo and seedling development in Arabidopsis thaliana. However, the regulatory mechanism of the FIE gene in postembryonic shoot development is not well understood. Silencing of Nicotiana benthamiana homologues of the FIE gene, NbFIE1 and NbFIE2, resulted in the enhanced outgrowth of axillary buds and the impairment of secondary xylem differentiation. RNA sequencing analysis found that one of the auxin-responsive GRETCHEN HAGEN 3 (GH3) family genes, NbGH3.6, was upregulated and maintained a high expression during the time course of silencing NbFIE genes. Chromatin immunoprecipiation (ChIP)-PCR results showed a lack of H3K27me3 marks on NbGH3.6 chromatin in NbFIE-silenced plants compared with negative control plants, indicating that NbGH3.6 was a direct target of NbFIE genes during postembryonic shoot development. Moreover, the free IAA content was reduced significantly in NbFIE-silenced plants, which might cause the enhanced outgrowth of axillary buds as well as impaired secondary xylem differentiation. These results clearly indicated that NbGH3.6 was a primary target of NbFIE genes during postembryonic shoot development, and NbFIE genes regulated axillary bud growth and secondary xylem formation through tuning endogenous auxin homeostasis, possibly by regulating the expression of the NbGH3.6 gene.; National Key Basic Research Program of China [2012CB114500]; National Natural Science Foundation of China [31270219]; SCI(E); PubMed; ARTICLE; hexq@pku.edu.cn; 8; 2207-2217; 67
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434951]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zeng, Jun,Ding, Qi,Fukuda, Hiroo,et al. Fertilization Independent Endosperm genes repress NbGH3.6 and regulate the auxin level during shoot development in Nicotiana benthamiana[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016.
APA	Zeng, Jun,Ding, Qi,Fukuda, Hiroo,&He, Xin-Qiang.(2016).Fertilization Independent Endosperm genes repress NbGH3.6 and regulate the auxin level during shoot development in Nicotiana benthamiana.JOURNAL OF EXPERIMENTAL BOTANY.
MLA	Zeng, Jun,et al."Fertilization Independent Endosperm genes repress NbGH3.6 and regulate the auxin level during shoot development in Nicotiana benthamiana".JOURNAL OF EXPERIMENTAL BOTANY (2016).