Yeast Two-Hybrid Screening for Proteins that Interact with the   Extracellular Domain of Amyloid Precursor Protein

doi:10.1007/s12264-016-0021-1

CORC > 北京大学 > 生命科学学院

	Yeast Two-Hybrid Screening for Proteins that Interact with the Extracellular Domain of Amyloid Precursor Protein
	Yu, You ; Li, Yinan ; Zhang, Yan
刊名	NEUROSCIENCE BULLETIN
	2016
关键词	Amyloid precursor protein Alzheimer&apos Yeast two-hybrid screening Cell death Pelizaeus-Merzbacher disease PELIZAEUS-MERZBACHER DISEASE APP-BINDING SURFACE FLRT3 CELL OUTGROWTH ADHESION NEURONS SYSTEM FE65 s disease
DOI	10.1007/s12264-016-0021-1
英文摘要	Alzheimer's disease (AD) is a neurodegenerative disorder in which amyloid beta plaques are a pathological characteristic. Little is known about the physiological functions of amyloid beta precursor protein (APP). Based on its structure as a type I transmembrane protein, it has been proposed that APP might be a receptor, but so far, no ligand has been reported. In the present study, 9 proteins binding to the extracellular domain of APP were identified using a yeast two-hybrid system. After confirming the interactions in the mammalian system, mutated PLP1, members of the FLRT protein family, and KCTD16 were shown to interact with APP. These proteins have been reported to be involved in Pelizaeus-Merzbacher disease (PMD) and axon guidance. Therefore, our results shed light on the mechanisms of physiological function of APP in AD, PMD, and axon guidance.; National Natural Science Foundation of China [81425009]; Beijing Natural Science Foundation, China [7142085]; Peking University Collaborative Fund, China [464-10606-00416]; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); ARTICLE; yanzhang@pku.edu.cn; 2; 171-176; 32
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434561]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yu, You,Li, Yinan,Zhang, Yan. Yeast Two-Hybrid Screening for Proteins that Interact with the Extracellular Domain of Amyloid Precursor Protein[J]. NEUROSCIENCE BULLETIN,2016.
APA	Yu, You,Li, Yinan,&Zhang, Yan.(2016).Yeast Two-Hybrid Screening for Proteins that Interact with the Extracellular Domain of Amyloid Precursor Protein.NEUROSCIENCE BULLETIN.
MLA	Yu, You,et al."Yeast Two-Hybrid Screening for Proteins that Interact with the Extracellular Domain of Amyloid Precursor Protein".NEUROSCIENCE BULLETIN (2016).