SUVH2 and SUVH9 Couple Two Essential Steps for Transcriptional Gene   Silencing in Arabidopsis

doi:10.1016/j.molp.2016.05.006

CORC > 北京大学 > 生命科学学院

	SUVH2 and SUVH9 Couple Two Essential Steps for Transcriptional Gene Silencing in Arabidopsis
	Jing, Yuqing ; Sun, Han ; Yuan, Wei ; Wang, Yue ; Li, Qi ; Liu, Yannan ; Li, Yan ; Qian, Weiqiang
刊名	MOLECULAR PLANT
	2016
关键词	epigenetics DNA methylation SUVH9 (At4g13460) MORC6 (At1g19100) transcriptional gene silencing chromatin remodeling DIRECTED DNA METHYLATION RNA-POLYMERASE V HISTONE ACETYLTRANSFERASE MUTANTS REVEALS NONCODING RNA POL IV DEMETHYLATION CHROMATIN PROTEIN FAMILY
DOI	10.1016/j.molp.2016.05.006
英文摘要	In Arabidopsis, an RNA-directed DNA methylation pathway (RdDM) is responsible for de novo establishment of DNA methylation and contributes to transcriptional gene silencing. Recently, the microrchidia (MORC)-type ATPases were shown to play essential roles in enforcing transcriptional gene silencing of a subset of genes and transposons by regulating the formation of higher-order chromatin architecture. However, how MORC proteins cooperate with the RdDM pathway components to regulate gene expression remains largely unclear. In this study, SUVH9 and MORC6 were identified from a screening of suppressors of idm1, which is a mutant defective in active DNA demethylation. SUVH9 and MORC6 are required for silencing of two reporter genes and some endogenous genes without enhancing DNA methylation levels. SUVH9, one of SU(VAR)3-9 homologs involved in RdDM, directly interacts with MORC6 and its two close homologs, MORC1 and MORC2. Similar to MORC6, SUVH9 and its homolog SUVH2 are required for heterochromatin condensation and formation of 3D chromatin architecture at SDC and Solo-LTR loci. We propose that SUVH2 and SUVH9 bind to the methylated DNA and facilitate the recruitment of a chromatin-remodeling complex to the target loci in association with MORC proteins.; National Natural Science Foundation of China [31571326, 31522005]; Thousand Youth Talents Program; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); ARTICLE; wqqian@pku.edu.cn; 8; 1156-1167; 9
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433622]
专题	生命科学学院
推荐引用方式 GB/T 7714	Jing, Yuqing,Sun, Han,Yuan, Wei,et al. SUVH2 and SUVH9 Couple Two Essential Steps for Transcriptional Gene Silencing in Arabidopsis[J]. MOLECULAR PLANT,2016.
APA	Jing, Yuqing.,Sun, Han.,Yuan, Wei.,Wang, Yue.,Li, Qi.,...&Qian, Weiqiang.(2016).SUVH2 and SUVH9 Couple Two Essential Steps for Transcriptional Gene Silencing in Arabidopsis.MOLECULAR PLANT.
MLA	Jing, Yuqing,et al."SUVH2 and SUVH9 Couple Two Essential Steps for Transcriptional Gene Silencing in Arabidopsis".MOLECULAR PLANT (2016).