RNF123 has an E3 ligase-independent function in RIG-I-like   receptor-mediated antiviral signaling

doi:10.15252/embr.201541703

CORC > 北京大学 > 生命科学学院

	RNF123 has an E3 ligase-independent function in RIG-I-like receptor-mediated antiviral signaling
	Wang, Shuai ; Yang, Yong-Kang ; Chen, Tao ; Zhang, Heng ; Yang, Wei-Wei ; Song, Sheng-Sheng ; Zhai, Zhong-He ; Chen, Dan-Ying
刊名	EMBO REPORTS
	2016
关键词	IFN-beta innate immunity MDA5 RIG-I RNF123 CYCLIC GMP-AMP INNATE IMMUNE-RESPONSE INDUCIBLE GENE-I UBIQUITIN LIGASE ADAPTER PROTEIN REGULATES PROTEOLYSIS NEGATIVE REGULATION CYTOSOLIC DNA G(1) PHASE CELL-CYCLE
DOI	10.15252/embr.201541703
英文摘要	Retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) are cytoplasmic sensors crucial for recognizing different species of viral RNAs, which triggers the production of type I interferons (IFNs) and inflammatory cytokines. Here, we identify RING finger protein 123 (RNF123) as a negative regulator of RIG-I and MDA5. Overexpression of RNF123 inhibits IFN-beta production triggered by Sendai virus (SeV) and encephalomyocarditis picornavirus (EMCV). Knockdown or knockout of endogenous RNF123 potentiates IFN-beta production triggered by SeV and EMCV, but not by the sensor of DNA viruses cGAS. RNF123 associates with RIG-I and MDA5 in both endogenous and exogenous cases in a viral infection-inducible manner. The SPRY and coiled-coil, but not the RING, domains of RNF123 are required for the inhibitory function. RNF123 interacts with the N-terminal CARD domains of RIG-I/MDA5 and competes with the downstream adaptor VISA/MAVS/IPS-1/Cardif for RIG-I/MDA5 CARD binding. These findings suggest that RNF123 functions as a novel inhibitor of innate antiviral signaling mediated by RIG-I and MDA5, a function that does not depend on its E3 ligase activity.; National Natural Science Foundation of China [31170822, 31470841]; SCI(E); PubMed; ARTICLE; dychen@pku.edu.cn; 8; 1155-1168; 17
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433321]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Shuai,Yang, Yong-Kang,Chen, Tao,et al. RNF123 has an E3 ligase-independent function in RIG-I-like receptor-mediated antiviral signaling[J]. EMBO REPORTS,2016.
APA	Wang, Shuai.,Yang, Yong-Kang.,Chen, Tao.,Zhang, Heng.,Yang, Wei-Wei.,...&Chen, Dan-Ying.(2016).RNF123 has an E3 ligase-independent function in RIG-I-like receptor-mediated antiviral signaling.EMBO REPORTS.
MLA	Wang, Shuai,et al."RNF123 has an E3 ligase-independent function in RIG-I-like receptor-mediated antiviral signaling".EMBO REPORTS (2016).