Antisense suppression of LOX3 gene expression in rice endosperm enhances   seed longevity

doi:10.1111/pbi.12277

CORC > 北京大学 > 生命科学学院

	Antisense suppression of LOX3 gene expression in rice endosperm enhances seed longevity
	Xu, Huibin ; Wei, Yidong ; Zhu, Yongsheng ; Lian, Ling ; Xie, Hongguang ; Cai, Qiuhua ; Chen, Qiushi ; Lin, Zhongping ; Wang, Zonghua ; Xie, Huaan ; Zhang, Jianfu
刊名	PLANT BIOTECHNOLOGY JOURNAL
	2015
关键词	seed longevity rice LOX3 artificial ageing SITE-SPECIFIC RECOMBINATION MASS-SPECTROMETRY PRODUCT SPECIFICITY TRANSGENIC PLANTS SOYBEAN SEEDS MARKER GENES LIPOXYGENASE PROTEOMICS ITRAQ TOOL
DOI	10.1111/pbi.12277
英文摘要	Lipid peroxidation plays a major role in seed longevity and viability. In rice grains, lipid peroxidation is catalyzed by the enzyme lipoxygenase 3 (LOX3). Previous reports showed that grain from the rice variety DawDam in which the LOX3 gene was deleted had less stale flavour after grain storage than normal rice. The molecular mechanism by which LOX3 expression is regulated during endosperm development remains unclear. In this study, we expressed a LOX3 antisense construct in transgenic rice (Oryza sativa L.) plants to down-regulate LOX3 expression in rice endosperm. The transgenic plants exhibited a marked decrease in LOX mRNA levels, normal phenotypes and a normal life cycle. We showed that LOX3 activity and its ability to produce 9-hydroperoxyoctadecadienoic acid (9-HPOD) from linoleic acid were significantly lower in transgenic seeds than in wild-type seeds by measuring the ultraviolet absorption of 9-HPOD at 234nm and by high-performance liquid chromatography. The suppression of LOX3 expression in rice endosperm increased grain storability. The germination rate of TS-91 (antisense LOX3 transgenic line) was much higher than the WT (29% higher after artificial ageing for 21days, and 40% higher after natural ageing for 12months). To our knowledge, this is the first report to demonstrate that decreased LOX3 expression can preserve rice grain quality during storage with no impact on grain yield, suggesting potential applications in agricultural production.; Hi-Tech Research and Development (863) Program of China [2014AA10A603, 2014AA10A604]; National Major Projects of Cultivated Transgenic New Crop Varieties Foundation of China [2008ZX001-006, 2011ZX001-006, 2011ZX001-004]; SCI(E); ARTICLE; huaanxie@163.com; jianfzhang@163.com; 4; 526-539; 13
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/420398]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, Huibin,Wei, Yidong,Zhu, Yongsheng,et al. Antisense suppression of LOX3 gene expression in rice endosperm enhances seed longevity[J]. PLANT BIOTECHNOLOGY JOURNAL,2015.
APA	Xu, Huibin.,Wei, Yidong.,Zhu, Yongsheng.,Lian, Ling.,Xie, Hongguang.,...&Zhang, Jianfu.(2015).Antisense suppression of LOX3 gene expression in rice endosperm enhances seed longevity.PLANT BIOTECHNOLOGY JOURNAL.
MLA	Xu, Huibin,et al."Antisense suppression of LOX3 gene expression in rice endosperm enhances seed longevity".PLANT BIOTECHNOLOGY JOURNAL (2015).