Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic beta- and alpha-Cells | |
Mohan, Ramkumar ; Mao, Yiping ; Zhang, Shungang ; Zhang, Yu-Wei ; Xu, Cheng-Ran ; Gradwohl, Gerard ; Tang, Xiaoqing | |
刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
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2015 | |
关键词 | GROWTH-FACTOR-II SWITCH-OFF HYPOTHESIS GLUCAGON-SECRETION CYTOKINE SIGNALING-3 INSULIN-RESISTANCE GLUCOSE-HOMEOSTASIS DIABETES-MELLITUS ROAD MAP PROLIFERATION APOPTOSIS |
DOI | 10.1074/jbc.M115.650705 |
英文摘要 | Insulin secreted from pancreatic beta-cells and glucagon secreted from pancreatic alpha-cells are the two major hormones working in the pancreas in an opposing manner to regulate and maintain a normal glucose homeostasis. How microRNAs (miRNAs), a population of non-coding RNAs so far demonstrated to be differentially expressed in various types of cells, regulate gene expression in pancreatic beta-cells and its closely associated alpha-cells is not completely clear. In this study, miRNA profiling was performed and compared between pancreatic beta-cells and their partner alpha-cells. One novel miRNA, miR-483, was identified for its highly differential expression in pancreatic beta-cells when compared to its expression in alpha-cells. Overexpression of miR-483 in beta-cells increased insulin transcription and secretion by targeting SOCS3, a member of suppressor of cytokine signaling family. In contrast, overexpression of miR-483 decreased glucagon transcription and secretion in alpha-cells. Moreover, overexpressed miR-483 protected against proinflammatory cytokine-induced apoptosis in beta-cells. This correlates with a higher expression level of miR-483 and the expanded beta-cell mass observed in the islets of prediabetic db/db mice. Together, our data suggest that miR-483 has opposite effects in alpha- and beta-cells by targeting SOCS3, and the imbalance of miR-483 and its targets may play a crucial role in diabetes pathogenesis.; National Institutes of Health [DK084166]; SCI(E); EI; PubMed; ARTICLE; xtang2@mtu.edu; 32; 19955-19966; 290 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/417254] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Mohan, Ramkumar,Mao, Yiping,Zhang, Shungang,et al. Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic beta- and alpha-Cells[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2015. |
APA | Mohan, Ramkumar.,Mao, Yiping.,Zhang, Shungang.,Zhang, Yu-Wei.,Xu, Cheng-Ran.,...&Tang, Xiaoqing.(2015).Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic beta- and alpha-Cells.JOURNAL OF BIOLOGICAL CHEMISTRY. |
MLA | Mohan, Ramkumar,et al."Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic beta- and alpha-Cells".JOURNAL OF BIOLOGICAL CHEMISTRY (2015). |
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