RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought   Tolerance in Maize

doi:10.1371/journal.pone.0143128

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	RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought Tolerance in Maize
	Liu, Ya ; Zhou, Miaoyi ; Gao, Zhaoxu ; Ren, Wen ; Yang, Fengling ; He, Hang ; Zhao, Jiuran
刊名	PLOS ONE
	2015
关键词	ACTIVATED PROTEIN-KINASE MEDIATES OSMOTIC-STRESS SIGNAL-TRANSDUCTION GENE FAMILY ZEA-MAYS TRANSGENIC ARABIDOPSIS ENVIRONMENTAL CUES DIFFERENTIAL GENE ABIOTIC STRESSES CANDIDATE GENES
DOI	10.1371/journal.pone.0143128
英文摘要	The mitogen-activated protein kinase (MAPK) cascade is an evolutionarily conserved signal transduction pathway that is involved in plant development and stress responses. As the first component of this phosphorelay cascade, mitogen-activated protein kinase kinase kinases (MAPKKKs) act as adaptors linking upstream signaling steps to the core MAPK cascade to promote the appropriate cellular responses; however, the functions of MAPKKKs in maize are unclear. Here, we identified 71 MAPKKK genes, of which 14 were novel, based on a computational analysis of the maize (Zea mays L.) genome. Using an RNA-seq analysis in the leaf, stem and root of maize under well-watered and drought-stress conditions, we identified 5,866 differentially expressed genes (DEGs), including 8 MAPKKK genes responsive to drought stress. Many of the DEGs were enriched in processes such as drought stress, abiotic stimulus, oxidation-reduction, and metabolic processes. The other way round, DEGs involved in processes such as oxidation, photosynthesis, and starch, proline, ethylene, and salicylic acid metabolism were clearly co-expressed with the MAPKKK genes. Furthermore, a quantitative real-time PCR (qRT-PCR) analysis was performed to assess the relative expression levels of MAPKKKs. Correlation analysis revealed that there was a significant correlation between expression levels of two MAPKKKs and relative biomass responsive to drought in 8 inbred lines. Our results indicate that MAPKKKs may have important regulatory functions in drought tolerance in maize.; National Transgenic Major Program of China [2014ZX0800303B]; Program of Beijing New Scientific Star [Z111105054511066]; SCI(E); PubMed; ARTICLE; hehang@pku.edu.cn; maizezhao@126.com; 11; e0143128; 10
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/416569]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Ya,Zhou, Miaoyi,Gao, Zhaoxu,et al. RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought Tolerance in Maize[J]. PLOS ONE,2015.
APA	Liu, Ya.,Zhou, Miaoyi.,Gao, Zhaoxu.,Ren, Wen.,Yang, Fengling.,...&Zhao, Jiuran.(2015).RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought Tolerance in Maize.PLOS ONE.
MLA	Liu, Ya,et al."RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought Tolerance in Maize".PLOS ONE (2015).