Inflating bacterial cells by increased protein synthesis

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	Inflating bacterial cells by increased protein synthesis
	Basan, Markus ; Zhu, Manlu ; Dai, Xiongfeng ; Warren, Mya ; Sevin, Daniel ; Wang, Yi-Ping ; Hwa, Terence
刊名	MOLECULAR SYSTEMS BIOLOGY
	2015
关键词	cell size cell division cellular DNA cell volume growth rate ESCHERICHIA-COLI K-12 SIZE CONTROL GROWTH-RATE CHROMOSOME-REPLICATION GENE-EXPRESSION INITIATION YEAST HOMEOSTASIS METABOLISM PHYSIOLOGY
英文摘要	Understanding how the homeostasis of cellular size and composition is accomplished by different organisms is an outstanding challenge in biology. For exponentially growing Escherichia coli cells, it is long known that the size of cells exhibits a strong positive relation with their growth rates in different nutrient conditions. Here, we characterized cell sizes in a set of orthogonal growth limitations. We report that cell size and mass exhibit positive or negative dependences with growth rate depending on the growth limitation applied. In particular, synthesizing large amounts of "useless" proteins led to an inversion of the canonical, positive relation, with slow growing cells enlarged 7- to 8-fold compared to cells growing at similar rates under nutrient limitation. Strikingly, this increase in cell size was accompanied by a 3- to 4-fold increase in cellular DNA content at slow growth, reaching up to an amount equivalent to similar to 8 chromosomes per cell. Despite drastic changes in cell mass and macromolecular composition, cellular dry mass density remained constant. Our findings reveal an important role of protein synthesis in cell division control.; SCI(E); PubMed; ARTICLE; basan@imsb.biol.ethz.ch; hwa@ucsd.edu; 10; 836; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/415839]
专题	生命科学学院
推荐引用方式 GB/T 7714	Basan, Markus,Zhu, Manlu,Dai, Xiongfeng,et al. Inflating bacterial cells by increased protein synthesis[J]. MOLECULAR SYSTEMS BIOLOGY,2015.
APA	Basan, Markus.,Zhu, Manlu.,Dai, Xiongfeng.,Warren, Mya.,Sevin, Daniel.,...&Hwa, Terence.(2015).Inflating bacterial cells by increased protein synthesis.MOLECULAR SYSTEMS BIOLOGY.
MLA	Basan, Markus,et al."Inflating bacterial cells by increased protein synthesis".MOLECULAR SYSTEMS BIOLOGY (2015).