Perturbation of the antigen-binding site and staphylococcal Protein   A-binding site of IgG before significant changes in global conformation   during denaturation: an equilibrium study

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	Perturbation of the antigen-binding site and staphylococcal Protein A-binding site of IgG before significant changes in global conformation during denaturation: an equilibrium study
	Wang, XD ; Luo, J ; Guo, ZQ ; Zhou, JM ; Tsou, CL
刊名	biochemical journal
	1997
关键词	INACTIVATION PRECEDES ADENYLATE KINASE RESOLUTION GUANIDINE FRAGMENT AUREUS
英文摘要	Although conformational perturbation of the active sites of many enzymes has been reported to precede global molecular conformational changes [Tsou (1993) Science 262, 380-381], little effort has been made to compare the susceptibility of the ligand-binding site of proteins and the protein molecules as a whole to perturbation by denaturants. Immunoglobulin is chosen in this study to address this problem. It is found that the variable and constant regions (Fv and Fc) of a monoclonal antibody of an IgG subclass against adenylate kinase lose their abilities to bind antigen and staphylococcal Protein A after treatment with guanidinium chloride concentrations considerably lower than those required to change the global conformation of the antibody as a whole, as detected by fluorescence and second-derivative UV absorption spectroscopy. These results indicate that both ligand-binding sites of the antibody concerned are more fragile than the molecule as a whole and that the Fv and Fc regions of the antibody molecule unfold sequentially during denaturation.; Biochemistry & Molecular Biology; SCI(E); 11; ARTICLE; 707-710; 325
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/401718]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, XD,Luo, J,Guo, ZQ,et al. Perturbation of the antigen-binding site and staphylococcal Protein A-binding site of IgG before significant changes in global conformation during denaturation: an equilibrium study[J]. biochemical journal,1997.
APA	Wang, XD,Luo, J,Guo, ZQ,Zhou, JM,&Tsou, CL.(1997).Perturbation of the antigen-binding site and staphylococcal Protein A-binding site of IgG before significant changes in global conformation during denaturation: an equilibrium study.biochemical journal.
MLA	Wang, XD,et al."Perturbation of the antigen-binding site and staphylococcal Protein A-binding site of IgG before significant changes in global conformation during denaturation: an equilibrium study".biochemical journal (1997).