miR-17-92 cluster accelerates adipocyte differentiation by negatively   regulating. tumor-suppressor Rb2/p130

doi:10.1073/pnas.0800178105

CORC > 北京大学 > 生命科学学院

	miR-17-92 cluster accelerates adipocyte differentiation by negatively regulating. tumor-suppressor Rb2/p130
	Wang, Qiang ; Li, Yan Chun ; Wang, Jinhua ; Kong, Juan ; Qi, Yuchen ; Quigg, Richard J. ; Li, Xinmin
刊名	proceedings of the national academy of sciences of the united states of america
	2008
关键词	ACTIVATED RECEPTOR-GAMMA E2F EXPRESSION PROTEINS P130 FAMILY DICER GENE
DOI	10.1073/pnas.0800178105
英文摘要	Adipogenesis involves cell proliferation and differentiation, both of which have been shown to be regulated by micro (mi)RNA. During mouse preadipocyte 3T3L1 cell differentiation, we found that miR-17-92, a miRNA cluster that promotes cell proliferation in various cancers, was significantly up-regulated at the clonal expansion stage of adipocyte differentiation. Stable transfection of 3T3L1 cells with miR-17-92 resulted in accelerated differentiation and increased triglyceride accumulation after hormonal stimulation. By using a luciferase reporter assay, we demonstrated that miR-17-92 directly targeted the 3' UTR region of Rb2/p130, accounting for subsequently reduced Rb2/p130 mRNA and protein quantities at the stage of clonal expansion. siRNA-mediated knock-down of Rb2/p130 at the same stage of clonal expansion recapitulated the phenotype of overexpression of miR-17-92 in the stably transfected 3T3L1 cells. These data indicate that miR-17-92 promotes adipocyte differentiation by targeting and negatively regulating Rb2/p130.; Multidisciplinary Sciences; SCI(E); 147; ARTICLE; 8; 2889-2894; 105
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/401367]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Qiang,Li, Yan Chun,Wang, Jinhua,et al. miR-17-92 cluster accelerates adipocyte differentiation by negatively regulating. tumor-suppressor Rb2/p130[J]. proceedings of the national academy of sciences of the united states of america,2008.
APA	Wang, Qiang.,Li, Yan Chun.,Wang, Jinhua.,Kong, Juan.,Qi, Yuchen.,...&Li, Xinmin.(2008).miR-17-92 cluster accelerates adipocyte differentiation by negatively regulating. tumor-suppressor Rb2/p130.proceedings of the national academy of sciences of the united states of america.
MLA	Wang, Qiang,et al."miR-17-92 cluster accelerates adipocyte differentiation by negatively regulating. tumor-suppressor Rb2/p130".proceedings of the national academy of sciences of the united states of america (2008).