Nuclear reconstitution of demembranated Orychophragmus violaceus sperm   in Xenopus laevis egg extracts

doi:10.1360/02yc9069

CORC > 北京大学 > 生命科学学院

	Nuclear reconstitution of demembranated Orychophragmus violaceus sperm in Xenopus laevis egg extracts
	Lu, P ; Ren, M ; Zhai, ZG
刊名	science in china series c life sciences
	2002
关键词	cell-free system nuclear reconstitution Orychophragmus violaceus demembranated sperm Xenopus laevis egg extracts CELL-FREE-EXTRACTS DNA-REPLICATION PROTEIN INVITRO
DOI	10.1360/02yc9069
英文摘要	The cell-free extracts from animal Xenopus laevis egg could induce chromatin decondensation and pronuclear formation from demembranated plant (Orychophragmus violaceus) sperm. The demembranated Orychophragmus violaceus sperm began to swell in 30 min incubation, and then were gradually decondensed. The reassembly of nuclear envelope in the reconstituted nuclei had been visualized by means of electron microscopy and fluorescent microscopy. Membrane vesicles fused to form the double envelope around the periphery of the decondensed chromatin. The morphology of the newly formed nucleus, with a double membrane, was similar to those nuclei after fertilization. Transmission electron microscope micrograph of the whole mount prepared nuclear matrix-lamina showed the reconstituted nucleus to be filled with a dense network.; Biology; SCI(E); EI; 0; 6; 623-630; 45
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/401106]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lu, P,Ren, M,Zhai, ZG. Nuclear reconstitution of demembranated Orychophragmus violaceus sperm in Xenopus laevis egg extracts[J]. science in china series c life sciences,2002.
APA	Lu, P,Ren, M,&Zhai, ZG.(2002).Nuclear reconstitution of demembranated Orychophragmus violaceus sperm in Xenopus laevis egg extracts.science in china series c life sciences.
MLA	Lu, P,et al."Nuclear reconstitution of demembranated Orychophragmus violaceus sperm in Xenopus laevis egg extracts".science in china series c life sciences (2002).