Biochemical characterization of the protein encoded by rice osRACD gene

doi:10.1080/10020070412331343571

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	Biochemical characterization of the protein encoded by rice osRACD gene
	Xu, YZ ; Mi, ZY ; Qu, H ; You, RL ; Wu, NH
刊名	progress in natural science
	2004
关键词	rice ( Oryza sativa L.) expression of osRACD gene photoperiod fertility transformation small molecular weight GTP-binding protein
DOI	10.1080/10020070412331343571
英文摘要	A recombinant plasmid pET-racd was first constructed by cloning osRACD, the development-regulating gene that controls photoperiod fertility transformation in the photoperiod sensitive genic mate sterile rice Nongken 58S, into a prokaryote expression vector pET28a( + ). It was then transformed into E. coli BL-21. Cutting with thrombin of the fusion protein extracted from transformants and PAGE separation yielded pure osRACD protein, which was further concentrated using ultrafiltration and renatured using glutathione oxidation/reduction refolding system for later functional study. As demonstrated by in vitro functional assay, the osRACD protein expressed in E. coli B-21 shows remarkable activity in binding GTP specifically and hydrolyzing it.; Materials Science, Multidisciplinary; Multidisciplinary Sciences; SCI(E); 0; ARTICLE; 4; 332-337; 14
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/400414]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, YZ,Mi, ZY,Qu, H,et al. Biochemical characterization of the protein encoded by rice osRACD gene[J]. progress in natural science,2004.
APA	Xu, YZ,Mi, ZY,Qu, H,You, RL,&Wu, NH.(2004).Biochemical characterization of the protein encoded by rice osRACD gene.progress in natural science.
MLA	Xu, YZ,et al."Biochemical characterization of the protein encoded by rice osRACD gene".progress in natural science (2004).