Biochemical characterization of the protein encoded by rice osRACD gene | |
Xu, YZ ; Mi, ZY ; Qu, H ; You, RL ; Wu, NH | |
刊名 | progress in natural science
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2004 | |
关键词 | rice ( Oryza sativa L.) expression of osRACD gene photoperiod fertility transformation small molecular weight GTP-binding protein |
DOI | 10.1080/10020070412331343571 |
英文摘要 | A recombinant plasmid pET-racd was first constructed by cloning osRACD, the development-regulating gene that controls photoperiod fertility transformation in the photoperiod sensitive genic mate sterile rice Nongken 58S, into a prokaryote expression vector pET28a( + ). It was then transformed into E. coli BL-21. Cutting with thrombin of the fusion protein extracted from transformants and PAGE separation yielded pure osRACD protein, which was further concentrated using ultrafiltration and renatured using glutathione oxidation/reduction refolding system for later functional study. As demonstrated by in vitro functional assay, the osRACD protein expressed in E. coli B-21 shows remarkable activity in binding GTP specifically and hydrolyzing it.; Materials Science, Multidisciplinary; Multidisciplinary Sciences; SCI(E); 0; ARTICLE; 4; 332-337; 14 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/400414] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Xu, YZ,Mi, ZY,Qu, H,et al. Biochemical characterization of the protein encoded by rice osRACD gene[J]. progress in natural science,2004. |
APA | Xu, YZ,Mi, ZY,Qu, H,You, RL,&Wu, NH.(2004).Biochemical characterization of the protein encoded by rice osRACD gene.progress in natural science. |
MLA | Xu, YZ,et al."Biochemical characterization of the protein encoded by rice osRACD gene".progress in natural science (2004). |
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