Temperature dependent protease activity and structural properties of   human HtrA2 protease

doi:10.1023/B:BIRY.0000033743.09806.1a

CORC > 北京大学 > 生命科学学院

	Temperature dependent protease activity and structural properties of human HtrA2 protease
	Zhang, XF ; Chang, ZY
刊名	biochemistry moscow
	2004
关键词	HtrA2 protease temperature proteolytic activity structural change HUMAN SERINE-PROTEASE HEAT-SHOCK PROTEASE ESCHERICHIA-COLI CONFORMATIONAL-CHANGES APOPTOSIS PROTEINS CASPASE ACTIVITY INHIBITOR CHAPERONE OMI/HTRA2 INTERACTS
DOI	10.1023/B:BIRY.0000033743.09806.1a
英文摘要	Human HtrA2 belongs to a new class of oligomeric serine protease, members of which are found in most organisms. Mature HtrA2 is released from mitochondria into the cytosol in response to apoptotic stimuli. In this report, the effect of temperature on proteolytic activity of HtrA2 and related structural properties were investigated. In the range from 25 to 55degreesC, the proteolytic activity of HtrA2 rapidly increased with temperature, and it drastically decreased at and over 60degreesC. Structural analysis using far-UV CD spectroscopy and gel filtration revealed no significant change in the secondary structure of HtrA2 from 25 to 70degreesC, or in the oligomeric size between 25 and 55degreesC. However, a significant change at the tertiary level, as examined using near-UV CD, was observed for HtrA2 in the range from 25 to 60degreesC. Differential scanning calorimetry indicated that HtrA2 exhibits a thermal transition beginning at around 61degreesC. The fluorescence intensity of ANS interacting with HtrA2 decreased with increasing temperature. HtrA2 was found to be able to complement DegP function at 44degreesC, indicating that HtrA2 could have protective functions in mitochondria.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000222756000013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); EI; 5; ARTICLE; 6; 687-692; 69
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/400228]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, XF,Chang, ZY. Temperature dependent protease activity and structural properties of human HtrA2 protease[J]. biochemistry moscow,2004.
APA	Zhang, XF,&Chang, ZY.(2004).Temperature dependent protease activity and structural properties of human HtrA2 protease.biochemistry moscow.
MLA	Zhang, XF,et al."Temperature dependent protease activity and structural properties of human HtrA2 protease".biochemistry moscow (2004).