Crystal structure of human cystatin D, a cysteine peptidase inhibitor   with restricted inhibition profile

doi:10.1074/jbc.M411914200

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	Crystal structure of human cystatin D, a cysteine peptidase inhibitor with restricted inhibition profile
	Alvarez-Fernandez, M ; Liang, YH ; Abrahamson, M ; Su, XD
刊名	journal of biological chemistry
	2005
关键词	3-DIMENSIONAL SOLUTION STRUCTURE EGG-WHITE CYSTATIN N-TERMINAL SEGMENT PROTEINASE-INHIBITOR CATHEPSIN-B CHICKEN CYSTATIN ASPARAGINYL ENDOPEPTIDASE PORPHYROMONAS-GINGIVALIS MAMMALIAN LEGUMAIN STEFIN-A
DOI	10.1074/jbc.M411914200
英文摘要	Cystatins are natural inhibitors of papain-like (family C1) and legumain-related (family C13) cysteine peptidases. Cystatin D is a type 2 cystatin, a secreted inhibitor found in human saliva and tear fluid. Compared with its homologues, cystatin D presents an unusual inhibition profile with a preferential inhibition cathepsin S > cathepsin H > cathepsin L and no inhibition of cathepsin B or pig legumain. To elucidate the structural reasons for this specificity, we have crystallized recombinant human Arg(26)-cystatin D and solved its structures at room temperature and at cryo conditions to 2.5- and 1.8-angstrom resolution, respectively. Human cystatin D presents the typical cystatin fold, with a five-stranded anti-parallel beta-sheet wrapped around a five-turn alpha-helix. The structures reveal differences in the peptidase-interacting regions when compared with other cystatins, providing plausible explanations for the restricted inhibitory specificity of cystatin D for some papain-like peptidases and its lack of reactivity toward legumain-related enzymes.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000228807200080&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); EI; 24; ARTICLE; 18; 18221-18228; 280
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/399383]
专题	生命科学学院
推荐引用方式 GB/T 7714	Alvarez-Fernandez, M,Liang, YH,Abrahamson, M,et al. Crystal structure of human cystatin D, a cysteine peptidase inhibitor with restricted inhibition profile[J]. journal of biological chemistry,2005.
APA	Alvarez-Fernandez, M,Liang, YH,Abrahamson, M,&Su, XD.(2005).Crystal structure of human cystatin D, a cysteine peptidase inhibitor with restricted inhibition profile.journal of biological chemistry.
MLA	Alvarez-Fernandez, M,et al."Crystal structure of human cystatin D, a cysteine peptidase inhibitor with restricted inhibition profile".journal of biological chemistry (2005).