Molecular cloning, expressional profiling, DNA binding and   trans-activation property studies of QRAP2 from Arabidopsis thalina

doi:10.1360/982005-706

CORC > 北京大学 > 生命科学学院

	Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thalina
	Wei, G ; Lei, J ; Gong, W ; Zhu, YX
刊名	科学通报英文版
	2005
关键词	QRAP2 stress DREB one-hybrid transcription factor TRANSCRIPTION FACTOR GENES LOW-TEMPERATURE STRESS DEHYDRATION ELEMENT DOMAIN PLANTS MICROARRAY ACTIVATORS TOLERANCE
DOI	10.1360/982005-706
英文摘要	Based upon analysis of data obtained from the ATH1 microarrays, a cDNA that was highly induced after drought treatment, was isolated from Arabidopsis seedlings. RT-PCR and Quantitative Real-Time (QRT)-PCR experiments showed that expression level of the gene increased significantly upon drought, UV, abscisic acid, high salinity and salicylic acid treatments. It was classified as a DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and contained a glutamine-rich region near its N terminus, we named it QRAP2 (for glutamine-rich AP2). Gel retardation assay revealed that QRAP2 was able to form a specific complex with the previously characterized DRE element while did not show any affinity to the GCC box or the mutant DRE box. When fused to the GAL4 DNA-binding domain, either fulllength QRAP2 or its N-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay with its C-terminus completely inactive. Our data indicate that QRAP2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream target genes in response to environmental stress, especially under drought conditions.; Multidisciplinary Sciences; SCI(E); 1; ARTICLE; 17; 1873-1878; 50
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/399145]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wei, G,Lei, J,Gong, W,et al. Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thalina[J]. 科学通报英文版,2005.
APA	Wei, G,Lei, J,Gong, W,&Zhu, YX.(2005).Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thalina.科学通报英文版.
MLA	Wei, G,et al."Molecular cloning, expressional profiling, DNA binding and trans-activation property studies of QRAP2 from Arabidopsis thalina".科学通报英文版 (2005).