Development of rabbit monoclonal and polyclonal antibodies for detection   of site-specific histone modifications and their application in   analyzing overall modification levels

doi:10.1038/sj.cr.7310063

CORC > 北京大学 > 生命科学学院

	Development of rabbit monoclonal and polyclonal antibodies for detection of site-specific histone modifications and their application in analyzing overall modification levels
	Guo, L ; Yin, BL ; Zhou, JL ; Li, XY ; Deng, XW
刊名	细胞研究英文版
	2006
关键词	historic modification site-specific antibody chromatin PEA PLASTOCYANIN GENE SACCHAROMYCES-CEREVISIAE H3 LYSINE-9 METHYLATION ACETYLATION HETEROCHROMATIN ARABIDOPSIS CHROMATIN LANGUAGE CELLS
DOI	10.1038/sj.cr.7310063
英文摘要	In addition to DNA sequence information, site-specific histone modifications are another important determinant of gene expression in a eukaryotic organism. We selected four modification sites in common histones that are known to significantly impact chromatin function and generated monoclonal or polyclonal antibodies that recognize each of those site-specific modifications. We used these antibodies to demonstrate that the site-specific histone modification levels remain relatively constant in different organs of the same organism. We also compared the levels of selected histone modifications among several representative organisms and found that site-specific modifications are highly variable among different organisms, providing new insight into the evolutionary divergence of specific histone modifications.; Cell Biology; SCI(E); 中国科技核心期刊(ISTIC); 0; ARTICLE; 5; 519-527; 16
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/398820]
专题	生命科学学院
推荐引用方式 GB/T 7714	Guo, L,Yin, BL,Zhou, JL,et al. Development of rabbit monoclonal and polyclonal antibodies for detection of site-specific histone modifications and their application in analyzing overall modification levels[J]. 细胞研究英文版,2006.
APA	Guo, L,Yin, BL,Zhou, JL,Li, XY,&Deng, XW.(2006).Development of rabbit monoclonal and polyclonal antibodies for detection of site-specific histone modifications and their application in analyzing overall modification levels.细胞研究英文版.
MLA	Guo, L,et al."Development of rabbit monoclonal and polyclonal antibodies for detection of site-specific histone modifications and their application in analyzing overall modification levels".细胞研究英文版 (2006).