Development of ELISA for metallothionein-II allows determination of   heavy metal pollution of fresh water

doi:10.2741/1954

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	Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water
	Chu, MM ; Guo, ZQ ; Muto, N ; Itoh, N ; Tanaka, K ; Ren, HW
刊名	frontiers in bioscience
	2006
关键词	metallothionein heavy metal pollution ELISA monoclonal antibody RAINBOW-TROUT HEPATIC METALLOTHIONEIN STONE LOACH CADMIUM INDUCTION EXPRESSION BINDING COPPER GENES PCR
DOI	10.2741/1954
英文摘要	Metallothionein (MT), a metal-binding protein induced primarily by heavy metals in vertebrates, is considered a biomarker for environmental heavy-metal contamination. To investigate heavy metal pollution in the freshwater environment, MT-I and MT-II were purified from livers of crucian carp (Carassius carassius) by gel exclusion chromatography and ion exchange chromatography. To detect the purified MT-II, a specific monoclonal antibody (mAb) against crucian carp MT-II was produced from the hybridoma strains by cell-cell fusion. By using Enzyme-Linked Immunosorbent Assay (ELISA) with this mAb, the purified crucian carp MT-II was detected with a high specificity and sensitivity. There was a good correlation between the amount of MT-II in carp livers and the concentration of heavy metals in water. ELISA was then used to evaluated the degree of heavy metal pollution in two freshwater systems. The results indicate that the MT-II content in carp liver tissue can be used as an indicator of environmental heavy-metal pollution.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000237382800013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Cell Biology; SCI(E); 9; 2113-2122; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/398387]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chu, MM,Guo, ZQ,Muto, N,et al. Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water[J]. frontiers in bioscience,2006.
APA	Chu, MM,Guo, ZQ,Muto, N,Itoh, N,Tanaka, K,&Ren, HW.(2006).Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water.frontiers in bioscience.
MLA	Chu, MM,et al."Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water".frontiers in bioscience (2006).