Analysis of transcription factor HY5 genomic binding sites revealed its   hierarchical role in light regulation of development

doi:10.1105/tpc.106.047688

CORC > 北京大学 > 生命科学学院

	Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development
	Lee, Jungeun ; He, Kun ; Stolc, Viktor ; Lee, Horim ; Figueroa, Pablo ; Gao, Ying ; Tongprasit, Waraporn ; Zhao, Hongyu ; Lee, Ilha ; Deng, XingWang
刊名	plant cell
	2007
关键词	MEDIATED PHOTOMORPHOGENIC GROWTH GENE-EXPRESSION CHROMATIN-IMMUNOPRECIPITATION ARABIDOPSIS-THALIANA PHENYLPROPANOID BIOSYNTHESIS MICROARRAY EXPERIMENTS SIGNAL-TRANSDUCTION CIRCADIAN CLOCK BZIP PROTEIN CHIP-CHIP
DOI	10.1105/tpc.106.047688
英文摘要	The transcription factor LONG HYPOCOTYL5 (HY5) acts downstream of multiple families of the photoreceptors and promotes photomorphogenesis. Although it is well accepted that HY5 acts to regulate target gene expression, in vivo binding of HY5 to any of its target gene promoters has yet to be demonstrated. Here, we used a chromatin immunoprecipitation procedure to verify suspected in vivo HY5 binding sites. We demonstrated that in vivo association of HY5 with promoter targets is not altered under distinct light qualities or during light-to-dark transition. Coupled with DNA chip hybridization using a high-density 60-nucleotide oligomer microarray that contains one probe for every 500 nucleotides over the entire Arabidopsis thaliana genome, we mapped genome-wide in vivo HY5 binding sites. This analysis showed that HY5 binds preferentially to promoter regions in vivo and revealed > 3000 chromosomal sites as putative HY5 binding targets. HY5 binding targets tend to be enriched in the early light-responsive genes and transcription factor genes. Our data thus support a model in which HY5 is a high hierarchical regulator of the transcriptional cascades for photomorphogenesis.; Biochemistry & Molecular Biology; Plant Sciences; Cell Biology; SCI(E); EI; 244; ARTICLE; 3; 731-749; 19
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/397850]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lee, Jungeun,He, Kun,Stolc, Viktor,et al. Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development[J]. plant cell,2007.
APA	Lee, Jungeun.,He, Kun.,Stolc, Viktor.,Lee, Horim.,Figueroa, Pablo.,...&Deng, XingWang.(2007).Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development.plant cell.
MLA	Lee, Jungeun,et al."Analysis of transcription factor HY5 genomic binding sites revealed its hierarchical role in light regulation of development".plant cell (2007).