GAMT2 encodes a methyltransferase of gibberellic acid that is involved   in seed maturation and germination in Arabidopsis

doi:10.1111/j.1672-9072.2007.00471.x

CORC > 北京大学 > 生命科学学院

	GAMT2 encodes a methyltransferase of gibberellic acid that is involved in seed maturation and germination in Arabidopsis
	Xing, Shufan ; Qin, Genji ; Shi, Yan ; Ma, Zhiqiang ; Chen, Zhangliang ; Gu, Hongya ; Qu, Li-Jia
刊名	journal of integrative plant biology
	2007
关键词	GAMT2 GA-deficient seed germination seed maturation Me-GA methyl acceptors SABATH methyltransferase CARBOXYL METHYLTRANSFERASE GENE-EXPRESSION JASMONIC ACID ENDOGENOUS GIBBERELLINS THALIANA PLANT MUTANTS DEFENSE ENZYME FAMILY
DOI	10.1111/j.1672-9072.2007.00471.x
英文摘要	Salicylic acid methyltransferase (SAMT), benzoic acid methyltransferase (BAMT) and theobromine methyltransferase (TH) (henceforth, SABATH) family proteins belong to a unique class of methyltransferase that can methylate small molecular compounds including indole-3-acidic acid (IAA), salicylic acid (SA) and jasmonic acid (JA), in plants. Here we report that the GAMT2 protein, which has 34.2% similarity with IAMT1 in the amino acid sequence, can methylate gibberellic acid (GA). Bioinformatics analysis suggests that GAMT2 may be able to methylate one molecule larger than SA. GAMT2 is predominantly expressed in the developing seed embryo and endosperm in Arabidopsis. During seed germination, the expression of GAMT2 decreases until the cotyledons expand out of the seed coat. Overexpression of GAMT2 in Arabidopsis resulted in multiple phenotypes, including dwarfism, retarded growth, late flowering, and reduced fertility, which are similar to the phenotypes of GA-deficient mutants. Seed germination assay showed that GAMT2 overexpression in plants was hypersensitive to GA biosynthesis inhibitor (ancymidol) and abscisic acid (ABA) treatments, whereas the GAMT2 null mutant (SALK_075450) was slightly insensitive to such treatments, suggesting that GAMT2 may methylate GA or ABA. Enzyme activity analysis indicated that GAMT2 was able to methylate GA(3) into Methyl-GA(3) in vitro, but could not methylate ABA. Microarray analysis on GAMT2 overexpression plants suggested that Methyl-GA may be an inactive form of GA in Arabidopsis. These data suggest that GAMT2 is involved in seed maturation and germination by modulating GA activity.; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 9; ARTICLE; 3; 368-381; 49
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/397834]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xing, Shufan,Qin, Genji,Shi, Yan,et al. GAMT2 encodes a methyltransferase of gibberellic acid that is involved in seed maturation and germination in Arabidopsis[J]. journal of integrative plant biology,2007.
APA	Xing, Shufan.,Qin, Genji.,Shi, Yan.,Ma, Zhiqiang.,Chen, Zhangliang.,...&Qu, Li-Jia.(2007).GAMT2 encodes a methyltransferase of gibberellic acid that is involved in seed maturation and germination in Arabidopsis.journal of integrative plant biology.
MLA	Xing, Shufan,et al."GAMT2 encodes a methyltransferase of gibberellic acid that is involved in seed maturation and germination in Arabidopsis".journal of integrative plant biology (2007).