In vitro development of mouse fetal germ cells into mature oocytes

doi:10.1530/REP-06-0378

CORC > 北京大学 > 生命科学学院

	In vitro development of mouse fetal germ cells into mature oocytes
	Shen, Wei ; Li, Lan ; Bai, Zhaodai ; Pan, Qingjie ; Ding, Mingxiao ; Deng, Hongkui
刊名	reproduction
	2007
关键词	EMBRYONIC STEM-CELLS PRIMORDIAL FOLLICLES INTERCELLULAR COMMUNICATION EPIGENETIC MODIFICATIONS EARLY FOLLICULOGENESIS FOLLICULAR DEVELOPMENT IMPRINTED GENES GAP-JUNCTIONS GROWTH MICE
DOI	10.1530/REP-06-0378
英文摘要	Little is known about the mechanisms underlying primordial follicular formation and the acquisition of competence to resume meiosis by growing oocytes. it is therefore important to establish an in vitro experimental model that allows one to study such mechanisms. Mouse follicular development has been studied in vitro over the past several years; however, no evidence has been presented showing that mature oocytes can be obtained from mouse fetal germ cells prior to the formation of primordial follicles. In this study, a method has been established to obtain mature oocytes from the mouse fetal germ cells at 16.5 days postcoitum (dpc). From the initiation of primordial follicular formation to the growth of early secondary follicles, ovarian tissues from 16.5 dpc fetal mice were cultured in vitro for 14 days. Subsequently, 678 intact secondary follicles were isolated from 182 mouse fetal ovaries and cultured for 12 days. A total of 141 oocytes inside antral follicles were matured in vitro, and 102 oocytes underwent germinal vesicle breakdown. We found that 97 oocytes were fertilized and 15 embryos were able to form morula-blastocysts. We also analyzed various genomic imprinting markers and showed that the erasure of genomic imprinting markers in the parental generation was also imposed on the oocytes that developed from fetal germ cells. Our results demonstrate that mouse fetal germ cells are able to form primordial follicles with ovarian cells, and that oocytes within the growing follicles are able to mature normally in vitro.; Developmental Biology; Reproductive Biology; SCI(E); 0; ARTICLE; 2; 223-231; 134
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/397748]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shen, Wei,Li, Lan,Bai, Zhaodai,et al. In vitro development of mouse fetal germ cells into mature oocytes[J]. reproduction,2007.
APA	Shen, Wei,Li, Lan,Bai, Zhaodai,Pan, Qingjie,Ding, Mingxiao,&Deng, Hongkui.(2007).In vitro development of mouse fetal germ cells into mature oocytes.reproduction.
MLA	Shen, Wei,et al."In vitro development of mouse fetal germ cells into mature oocytes".reproduction (2007).