The model plant Medicago truncatula exhibits biparental plastid   inheritance

doi:10.1093/pcp/pcm170

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	The model plant Medicago truncatula exhibits biparental plastid inheritance
	Matsushima, Ryo ; Hu, Yingchun ; Toyoda, Kazuhiro ; Sodmergen ; Sakamoto, Wataru
刊名	plant and cell physiology
	2008
关键词	biparental inheritance Medicago truncatula model plant plastid pollen grain PISUM-SATIVUM-L CYTOPLASMIC INHERITANCE MITOCHONDRIAL-DNA PATERNAL INHERITANCE MATERNAL INHERITANCE GENERATIVE CELLS INTRAMITOCHONDRIAL FIBERS TRANSGENE CONTAINMENT POLLEN DEVELOPMENT VEGETATIVE CELLS
DOI	10.1093/pcp/pcm170
英文摘要	The plastid, which originated from the endosymbiosis of a cyanobacterium, contains its own plastid DNA (ptDNA) that exhibits a unique mode of inheritance. Approximately 80 of angiosperms show maternal inheritance, whereas the remainder exhibit biparental inheritance of ptDNA. Here we studied ptDNA inheritance in the model legume, Medicago truncatula. Cytological analysis of mature pollen with DNA-specific fluorescent dyes suggested that M. truncatula is one of the few model plants potentially showing biparental inheritance of ptDNA. We further examined pollen by electron microscopy and revealed that the generative cell (a mother of sperm cells) indeed has many DNA-containing plastids. To confirm biparental inheritance genetically, we crossed two ecotypes (Jemalong A17 and A20), and the transmission mode of ptDNA was investigated by a PCR-assisted polymorphism. Consistent with the cytological observations, the majority of F(1) plants possessed ptDNAs from both parents. Interestingly, cotyledons of F(1) plants tended to retain a biparental ptDNA population, while later emergent leaves tended to be uniparental with either one of the parental plastid genotypes. Biparental transmission was obvious in the F(2) population, in which all plants showed homoplasmy with either a paternal or a maternal plastid genotype. Collectively, these data demonstrated that M. truncatula is biparental for ptDNA transmission and thus can be an excellent model to study plastid genetics in angiosperms.; Plant Sciences; Cell Biology; SCI(E); 0; ARTICLE; 1; 81-91; 49
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/397559]
专题	生命科学学院
推荐引用方式 GB/T 7714	Matsushima, Ryo,Hu, Yingchun,Toyoda, Kazuhiro,et al. The model plant Medicago truncatula exhibits biparental plastid inheritance[J]. plant and cell physiology,2008.
APA	Matsushima, Ryo,Hu, Yingchun,Toyoda, Kazuhiro,Sodmergen,&Sakamoto, Wataru.(2008).The model plant Medicago truncatula exhibits biparental plastid inheritance.plant and cell physiology.
MLA	Matsushima, Ryo,et al."The model plant Medicago truncatula exhibits biparental plastid inheritance".plant and cell physiology (2008).