Mitochondrial dynamics in plant male gametophyte visualized by   fluorescent live imaging

doi:10.1093/pcp/pcn084

CORC > 北京大学 > 生命科学学院

	Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging
	Matsushima, Ryo ; Hamamura, Yuki ; Higashiyama, Tetsuya ; Arimura, Shin-ichi ; Sodmergen ; Tsutsumi, Nobuhiro ; Sakamoto, Wataru
刊名	plant and cell physiology
	2008
关键词	Arabidopsis thaliana fertilization fluorescent protein mitochondria pollen grain sperm cell ARABIDOPSIS-THALIANA CYTOPLASMIC INHERITANCE PLUMBAGO-ZEYLANICA POLLEN TUBES INTRAMITOCHONDRIAL FIBERS TRANSGENE CONTAINMENT PATERNAL INHERITANCE DNA CHARACTERISTICS SPERM MITOCHONDRIA PLASTID DNA
DOI	10.1093/pcp/pcn084
英文摘要	Visualization of organelles in living cells is a powerful method for studying their dynamic behavior. Here we attempted to visualize mitochondria in angiosperm male gametophyte (pollen grain from Arabidopsis thaliana) that are composed of one vegetative cell (VC) and two sperm cells (SCs). Combination of mitochondria-targeted fluorescent proteins with VC- or SC-specific expression allowed us to observe the precise number and dynamic behavior of mitochondria in the respective cell types. Furthermore, live imaging of SC mitochondria during double fertilization confirmed previous observations, demonstrated by electron microscopy in other species, that sperm mitochondria enter into the egg and central cells. We also attempted to visualize mutant mitochondria that were elongated due to a defect in mitochondrial division. This mutant phenotype was indeed detectable in VC mitochondria of a heterozygous F(1) plant, suggesting active mitochondrial division in male gametophyte. Finally, we performed mutant screening and isolated a putative mitochondrial protein transport mutant whose phenotype was detectable only in haploid cells. The transgenic materials presented in this work are useful not only for live imaging but also for studying mitochondrial functions by mutant analysis.; Plant Sciences; Cell Biology; SCI(E); 23; ARTICLE; 7; 1074-1083; 49
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/397316]
专题	生命科学学院
推荐引用方式 GB/T 7714	Matsushima, Ryo,Hamamura, Yuki,Higashiyama, Tetsuya,et al. Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging[J]. plant and cell physiology,2008.
APA	Matsushima, Ryo.,Hamamura, Yuki.,Higashiyama, Tetsuya.,Arimura, Shin-ichi.,Sodmergen.,...&Sakamoto, Wataru.(2008).Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging.plant and cell physiology.
MLA	Matsushima, Ryo,et al."Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging".plant and cell physiology (2008).