DSCAM functions as a netrin receptor in commissural axon pathfinding

doi:10.1073/pnas.0811083106

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	DSCAM functions as a netrin receptor in commissural axon pathfinding
	Liu, Guofa ; Li, Weiquan ; Wang, Lei ; Kar, Amar ; Guan, Kun-Liang ; Rao, Yi ; Wu, Jane Y.
刊名	proceedings of the national academy of sciences of the united states of america
	2009
关键词	neurite outgrowth commissural axon guidance neuronal guidance receptor signal transduction FOCAL ADHESION KINASE C-ELEGANS COLORECTAL-CANCER NERVOUS-SYSTEM TRANSMEMBRANE PROTEIN GUIDANCE RECEPTOR GENETIC-ANALYSIS DROSOPHILA DCC UNC-5
DOI	10.1073/pnas.0811083106
英文摘要	Down syndrome cell adhesion molecule (DSCAM) is required for axon guidance and dendrite arborization. How DSCAM functions in vertebrates is not well understood. Here we show that DSCAM is expressed on commissural axons and interacts with Netrin-1, a prototypical guidance cue for commissural axons. The knockdown of DSCAM by specific siRNA or blockage of DSCAM signaling by overexpression of a mutant lacking its intracellular domain inhibits netrin-induced axon outgrowth and commissural axon turning in vitro. SiRNA-mediated knockdown of DSCAM in ovo causes defects in commissural axon projection and pathfinding. In transfected cells, DSCAM by itself, in the absence of DCC, is capable of mediating netrin signaling in activating phosphorylation of Fyn and Pak1. These findings demonstrate an essential role of vertebrate DSCAM in axon guidance, indicating that DSCAM functions as a receptor of netrin-1. Our data suggest previously unexpected complexity in receptors that mediate vertebrate netrin signaling.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000263652900086&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 73; ARTICLE; 8; 2951-2956; 106
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/396681]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Guofa,Li, Weiquan,Wang, Lei,et al. DSCAM functions as a netrin receptor in commissural axon pathfinding[J]. proceedings of the national academy of sciences of the united states of america,2009.
APA	Liu, Guofa.,Li, Weiquan.,Wang, Lei.,Kar, Amar.,Guan, Kun-Liang.,...&Wu, Jane Y..(2009).DSCAM functions as a netrin receptor in commissural axon pathfinding.proceedings of the national academy of sciences of the united states of america.
MLA	Liu, Guofa,et al."DSCAM functions as a netrin receptor in commissural axon pathfinding".proceedings of the national academy of sciences of the united states of america (2009).