Development of three Drosophila melanogaster strains with different   sensitivity to volatile anesthetics

doi:10.3760/cma.j.issn.0366-6999.2009.05.014

CORC > 北京大学 > 生命科学学院

	Development of three Drosophila melanogaster strains with different sensitivity to volatile anesthetics
	Liu Jin ; Hu Zhao-yang ; Ye Qi-quan ; Dai Shuo-hua
刊名	Chinese Medical Journal
	2009
关键词	drosophila melanogaster sensitivity median effective doses volatile anesthetics sevoflurane SEROTONIN TRANSPORTER GENERAL-ANESTHESIA EXPRESSION CLONING MUTANTS
DOI	10.3760/cma.j.issn.0366-6999.2009.05.014
英文摘要	Background The mechanisms of action for volatile anesthetics remain unknown for centuries partly owing to the insufficient or ineffective research models. We designed this study to develop three strains derived from a wild-type Drosophila melanogaster with different sensitivities to volatile anesthetics, which may ultimately facilitate molecular and genetic studies of the mechanism involved. Methods Median effective doses (ED(50)) of sevoflurane in seven-day-old virgin female and male wild-type Drosophila melanogaster were determined. The sensitive males and females of percentile 6-10 were cultured for breeding sensitive offspring (Si). So did median ones of percentile 48-52 for breeding median offspring (M(1)), resistant ones of percentile 91-95 for breeding resistant offspring (RI). Process was repeated through 31 generations, in the 37th generation, S(37), M(37) and R(37) were used to determine ED(50) for enflurane, isoflurane, sevoflurane, desflurane, halothane, methoxyflurane, chloroform and trichloroethylene, then ED(50) values were correlated with minimum alveolar concentration (MAC) values in human. Results From a wild-type Drosophila melanogaster we were able to breed three strains with high, median and low sevoflurane requirements. The ratio of sevoflurane requirements of three strains were 1.20:1.00:0.53 for females and 1.22:1.00:0.72 for males. Strains sensitive, median and resistant to sevoflurane were also sensitive, median and resistant to other volatile anesthetics. For eight anesthetics, ED(50) values in three strains correlated directly with MAC values in human. Conclusions Three Drosophila melanogaster strains with high, median and low sensitivity to volatile anesthetics, but with same hereditary background were developed. The ED(50) are directly correlated with MAC in human for eight volatile anesthetics.; Medicine, General & Internal; SCI(E); 中国科技核心期刊(ISTIC); 2; ARTICLE; 5; 561-565; 122
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/396629]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu Jin,Hu Zhao-yang,Ye Qi-quan,et al. Development of three Drosophila melanogaster strains with different sensitivity to volatile anesthetics[J]. Chinese Medical Journal,2009.
APA	Liu Jin,Hu Zhao-yang,Ye Qi-quan,&Dai Shuo-hua.(2009).Development of three Drosophila melanogaster strains with different sensitivity to volatile anesthetics.Chinese Medical Journal.
MLA	Liu Jin,et al."Development of three Drosophila melanogaster strains with different sensitivity to volatile anesthetics".Chinese Medical Journal (2009).