Disassembly intermediates of RbsD protein remain oligomeric despite the   loss of an intact secondary structure

doi:10.1007/s11427-009-0141-1

CORC > 北京大学 > 生命科学学院

	Disassembly intermediates of RbsD protein remain oligomeric despite the loss of an intact secondary structure
	Feng YongJun ; Zhang Meng ; Hu MingXi ; Zheng Jie ; Jiao WangWang ; Chang ZengYi
刊名	science in china series c life sciences
	2009
关键词	RbsD oligomeric protein oligomeric intermediate refolding reassembly DIMERIC INTERMEDIATE CRYSTAL-STRUCTURES MECHANISM TRYPSIN REPRESSOR SUBUNITS DOMAIN ELECTROPHORESIS CONFORMATION DIMERIZATION
DOI	10.1007/s11427-009-0141-1
英文摘要	Many proteins exist as homo-oligomers in living organisms wherein the change of oligomeric status apparently serves as an effective means for modulating their biological activities. We have previously reported that the homo-decameric RbsD from Escherichia coli undergoes stepwise disassembly and non-stepwise reassembly. Here the structural status of the urea-induced RbsD disassembly intermediates was examined, mainly using urea-containing polyacrylamide gel electrophoresis and chemical cross-linking. Such intermediates were found to remain oligomeric while losing their intact secondary structures. Such disassembly intermediates were able to effectively refold when the concentration of the urea denaturant was reduced to a lower level, or to refold/reassemble into the native decamers when urea was completely removed, as detected by non-denaturing polyacrylamide gel electrophoresis. These novel observations strongly suggest that the assembly of oligomeric proteins may occur before the completion of subunit folding.; Biology; SCI(E); 4; ARTICLE; 11; 997-1002; 52
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/396239]
专题	生命科学学院
推荐引用方式 GB/T 7714	Feng YongJun,Zhang Meng,Hu MingXi,et al. Disassembly intermediates of RbsD protein remain oligomeric despite the loss of an intact secondary structure[J]. science in china series c life sciences,2009.
APA	Feng YongJun,Zhang Meng,Hu MingXi,Zheng Jie,Jiao WangWang,&Chang ZengYi.(2009).Disassembly intermediates of RbsD protein remain oligomeric despite the loss of an intact secondary structure.science in china series c life sciences.
MLA	Feng YongJun,et al."Disassembly intermediates of RbsD protein remain oligomeric despite the loss of an intact secondary structure".science in china series c life sciences (2009).