Transcriptome profiling defines a novel regulon modulated by the   LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa

doi:10.1093/nar/gkp828

CORC > 北京大学 > 生命科学学院

	Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa
	Tian, Zhe-Xian ; Fargier, Emilie ; Mac Aogain, Micheal ; Adams, Claire ; Wang, Yi-Ping ; O&apos ; Gara, Fergal
刊名	核酸研究
	2009
关键词	MULTIDRUG EFFLUX SYSTEM DIFFERENTIAL SELECTION XENOBIOTIC REDUCTASES OPRN RESISTANCE EXPRESSION PROTEINS PUMPS OVEREXPRESSION IDENTIFICATION
DOI	10.1093/nar/gkp828
英文摘要	The LysR-family regulator MexT modulates the expression of the MexEF-OprN efflux system in the human pathogen Pseudomonas aeruginosa. Recently, we demonstrated that MexT regulates certain virulence phenotypes, including the type-three secretion system and early attachment independent of its role in regulating MexEF-OprN. In this study, transcriptome profiling was utilized to investigate the global nature of MexT regulation in P. aeruginosa PAO1 and an isogenic mexEF mutant. Twelve genes of unknown function were highly induced by overexpressing MexT independent of MexEF-OprN. A well-conserved DNA motif was identified in the upstream regulatory region of nine of these genes and upstream of mexE. Reporter fusion analysis demonstrated that the expression of the genes was significantly induced by MexT in P. aeruginosa and a heterogenous Escherichia coli strain and that the conserved sequence was required for this induction. The conserved DNA motif was further characterized as the MexT binding site by site-directed mutagenesis and electrophoretic mobility shift assays. Genes containing this conserved regulatory sequence were identified across other Pseudomonas species, and their expression was activated by MexT. Thus, a novel regulon directly modulated by MexT, that includes but is independent of mexEF-oprN, has been identified.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000272935000026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); 35; ARTICLE; 22; 7546-7559; 37
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/396149]
专题	生命科学学院
推荐引用方式 GB/T 7714	Tian, Zhe-Xian,Fargier, Emilie,Mac Aogain, Micheal,et al. Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa[J]. 核酸研究,2009.
APA	Tian, Zhe-Xian.,Fargier, Emilie.,Mac Aogain, Micheal.,Adams, Claire.,Wang, Yi-Ping.,...&Gara, Fergal.(2009).Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa.核酸研究.
MLA	Tian, Zhe-Xian,et al."Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa".核酸研究 (2009).