Genomic Characterization of Severe Acute Respiratory Syndrome-Related   Coronavirus in European Bats and Classification of Coronaviruses Based   on Partial RNA-Dependent RNA Polymerase Gene Sequences

doi:10.1128/JVI.00650-10

CORC > 北京大学 > 生命科学学院

	Genomic Characterization of Severe Acute Respiratory Syndrome-Related Coronavirus in European Bats and Classification of Coronaviruses Based on Partial RNA-Dependent RNA Polymerase Gene Sequences
	Drexler, Jan Felix ; Gloza-Rausch, Florian ; Glende, Joerg ; Corman, Victor Max ; Muth, Doreen ; Goettsche, Matthias ; Seebens, Antje ; Niedrig, Matthias ; Pfefferle, Susanne ; Yordanov, Stoian ; Zhelyazkov, Lyubomir ; Hermanns, Uwe ; Vallo, Peter ; Lukashev, Alexander ; Mueller, Marcel Alexander ; Deng, Hongkui ; Herrler, Georg ; Drosten, Christian
刊名	病毒学杂志
	2010
关键词	CROSS-SPECIES TRANSMISSION SARS-LIKE CORONAVIRUSES RESERVOIR HOSTS HORSESHOE BATS VIRUS IDENTIFICATION PROTEIN CHINA INFECTIONS PREVALENCE
DOI	10.1128/JVI.00650-10
英文摘要	Bats may host emerging viruses, including coronaviruses (CoV). We conducted an evaluation of CoV in rhinolophid and vespertilionid bat species common in Europe. Rhinolophids carried severe acute respiratory syndrome (SARS)-related CoV at high frequencies and concentrations (26% of animals are positive; up to 2.4 x 108 copies per gram of feces), as well as two Alphacoronavirus clades, one novel and one related to the HKU2 clade. All three clades present in Miniopterus bats in China (HKU7, HKU8, and 1A related) were also present in European Miniopterus bats. An additional novel Alphacoronavirus clade (bat CoV [BtCoV]/BNM98-30) was detected in Nyctalus leisleri. A CoV grouping criterion was developed by comparing amino acid identities across an 816-bp fragment of the RNA-dependent RNA polymerases (RdRp) of all accepted mammalian CoV species (RdRp-based grouping units [RGU]). Criteria for defining separate RGU in mammalian CoV were a > 4.8% amino acid distance for alphacoronaviruses and a > 6.3% distance for betacoronaviruses. All the abovementioned novel clades represented independent RGU. Strict associations between CoV RGU and host bat genera were confirmed for six independent RGU represented simultaneously in China and Europe. A SARSrelated virus (BtCoV/BM48-31/Bulgaria/2008) from a Rhinolophus blasii (Rhi bla) bat was fully sequenced. It is predicted that proteins 3b and 6 were highly divergent from those proteins in all known SARS-related CoV. Open reading frame 8 (ORF8) was surprisingly absent. Surface expression of spike and staining with sera of SARS survivors suggested low antigenic overlap with SARS CoV. However, the receptor binding domain of SARS CoV showed higher similarity with that of BtCoV/BM48-31/Bulgaria/2008 than with that of any Chinese bat-borne CoV. Critical spike domains 472 and 487 were identical and similar, respectively. This study underlines the importance of assessments of the zoonotic potential of widely distributed bat-borne CoV.; Virology; SCI(E); 57; ARTICLE; 21; 11336-11349; 84
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/395337]
专题	生命科学学院
推荐引用方式 GB/T 7714	Drexler, Jan Felix,Gloza-Rausch, Florian,Glende, Joerg,et al. Genomic Characterization of Severe Acute Respiratory Syndrome-Related Coronavirus in European Bats and Classification of Coronaviruses Based on Partial RNA-Dependent RNA Polymerase Gene Sequences[J]. 病毒学杂志,2010.
APA	Drexler, Jan Felix.,Gloza-Rausch, Florian.,Glende, Joerg.,Corman, Victor Max.,Muth, Doreen.,...&Drosten, Christian.(2010).Genomic Characterization of Severe Acute Respiratory Syndrome-Related Coronavirus in European Bats and Classification of Coronaviruses Based on Partial RNA-Dependent RNA Polymerase Gene Sequences.病毒学杂志.
MLA	Drexler, Jan Felix,et al."Genomic Characterization of Severe Acute Respiratory Syndrome-Related Coronavirus in European Bats and Classification of Coronaviruses Based on Partial RNA-Dependent RNA Polymerase Gene Sequences".病毒学杂志 (2010).