PDIp is a major intracellular oestrogen-storage protein that modulates   tissue levels of oestrogen in the pancreas

doi:10.1042/BJ20120868

CORC > 北京大学 > 生命科学学院

	PDIp is a major intracellular oestrogen-storage protein that modulates tissue levels of oestrogen in the pancreas
	Fu, Xinmiao ; Wang, Pan ; Fukui, Masayuki ; Long, Cheng ; Yin, Linxiang ; Choi, Hye Joung ; Zhu, Bao Ting
刊名	biochemical journal
	2012
关键词	17 beta-oestradiol (E-2) oestrogen oestrogen receptor pancreas pancreas-specific protein disulfide isomerase (PDIp) ESTRADIOL-BINDING PROTEIN DISULFIDE-ISOMERASE RECEPTOR-ALPHA INSULIN-SECRETION EXOCRINE PANCREAS TARGET-CELLS ACINAR-CELLS BETA-CELLS CANCER LOCALIZATION
DOI	10.1042/BJ20120868
英文摘要	E-2 (17 beta-oestradiol), a female sex hormone, has important biological functions in a woman's body. The pancreas, often considered a non-classical E-2-targeting organ, is known to be functionally regulated by E-2, but little is known about how oestrogen actions are regulated in this organ. In the present study we report that PDIp (pancreas-specific protein disulfide isomerase), a protein-folding catalyst, can act as a major intracellular E-2 storage protein in a rat model to modulate the pancreatic tissue level, metabolism and action of E-2. The purified endogenous PDIp from both rat and human pancreatic tissues can bind E-2 with a K-d value of approximately 150 nM. The endogenous PDIp-bound E-2 accounts for over 80% of the total protein-bound E-2 present in rat and human pancreatic tissues, and this binding protects E-2 from metabolic disposition and prolongs its duration of action. Importantly, we showed in ovariectomized female rats that the E2 level in the pancreas reaches its highest level (9-fold increase over its basal level) at 24-48 h after a single injection of E-2, and even at 96 h its level is still approximately 5-fold higher. In contrast, the E-2 level in the uterus quickly returns to its basal level at 48 h after reaching its maximal level (approximately 2-fold increase) at 24 h. Taken together, these results show for the first time that PDIp is a predominant intracellular oestrogen storage protein in the pancreas, which offers novel mechanistic insights into the accumulation and action of oestrogen inside pancreatic cells.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000309489600012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); 1; ARTICLE; 115-123; 447
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/392885]
专题	生命科学学院
推荐引用方式 GB/T 7714	Fu, Xinmiao,Wang, Pan,Fukui, Masayuki,et al. PDIp is a major intracellular oestrogen-storage protein that modulates tissue levels of oestrogen in the pancreas[J]. biochemical journal,2012.
APA	Fu, Xinmiao.,Wang, Pan.,Fukui, Masayuki.,Long, Cheng.,Yin, Linxiang.,...&Zhu, Bao Ting.(2012).PDIp is a major intracellular oestrogen-storage protein that modulates tissue levels of oestrogen in the pancreas.biochemical journal.
MLA	Fu, Xinmiao,et al."PDIp is a major intracellular oestrogen-storage protein that modulates tissue levels of oestrogen in the pancreas".biochemical journal (2012).