HSCARG Inhibits NADPH Oxidase Activity through Regulation of the   Expression of p47phox

doi:10.1371/journal.pone.0059301

CORC > 北京大学 > 生命科学学院

	HSCARG Inhibits NADPH Oxidase Activity through Regulation of the Expression of p47phox
	Xiao, Weichun ; Peng, Yanyan ; Liu, Yong ; Li, Zhi ; Li, Senlin ; Zheng, Xiaofeng
刊名	plos one
	2013
关键词	NF-KAPPA-B SENSOR PROTEIN TUMOR-CELLS ACTIVATION DIPHENYLENEIODONIUM MODULATION INDUCTION APOPTOSIS INCREASE ASSAY
DOI	10.1371/journal.pone.0059301
英文摘要	Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase catalyzes the transfer of electrons from NADPH to O-2, which is the main source of reactive oxygen species (ROS) in nonphagocytic cells. Excess ROS are toxic; therefore, keeping ROS in homeostasis in cells can protect cells from oxidative damage. It is meaningful to further understand the molecular mechanism by which ROS homeostasis is mediated. Human protein HSCARG is a newly identified oxidative sensor and a negative regulator of NF-kappa B. Here, we find that HSCARG represses the cellular ROS generation through inhibiting mRNA and protein expression of p47phox, a subunit of NADPH oxidase. In contrast, shRNA-mediated HSCARG knockdown increases endogenous p47phox expression level. And HSCARG has no obvious effect on ROS production in p47phox-depleted cells. Furthermore, HSCARG regulates p47phox through inhibition of NF-kappa B activity. Our findings identify HSCARG as a novel regulator in regulation of the activity of NADPH oxidase and ROS homeostasis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000317562100101&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 2; ARTICLE; 3; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/391948]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xiao, Weichun,Peng, Yanyan,Liu, Yong,et al. HSCARG Inhibits NADPH Oxidase Activity through Regulation of the Expression of p47phox[J]. plos one,2013.
APA	Xiao, Weichun,Peng, Yanyan,Liu, Yong,Li, Zhi,Li, Senlin,&Zheng, Xiaofeng.(2013).HSCARG Inhibits NADPH Oxidase Activity through Regulation of the Expression of p47phox.plos one.
MLA	Xiao, Weichun,et al."HSCARG Inhibits NADPH Oxidase Activity through Regulation of the Expression of p47phox".plos one (2013).