Coordination of bacterial proteome with metabolism by cyclic AMP   signalling

doi:10.1038/nature12446

CORC > 北京大学 > 生命科学学院

	Coordination of bacterial proteome with metabolism by cyclic AMP signalling
	You, Conghui ; Okano, Hiroyuki ; Hui, Sheng ; Zhang, Zhongge ; Kim, Minsu ; Gunderson, Carl W. ; Wang, Yi-Ping ; Lenz, Peter ; Yan, Dalai ; Hwa, Terence
刊名	nature
	2013
关键词	LAC OPERON EXPRESSION ESCHERICHIA-COLI CATABOLITE REPRESSION ADENYLATE-CYCLASE PHOSPHOTRANSFERASE SYSTEM TRANSIENT REPRESSION BETA-GALACTOSIDASE GENE-EXPRESSION DIAUXIC GROWTH GLUCOSE
DOI	10.1038/nature12446
英文摘要	The cyclic AMP (cAMP)-dependent catabolite repression effect in Escherichia coli is among the most intensely studied regulatory processes in biology. However, the physiological function(s) of cAMP signalling and its molecular triggers remain elusive. Here we use a quantitative physiological approach to show that cAMP signalling tightly coordinates the expression of catabolic proteins with biosynthetic and ribosomal proteins, in accordance with the cellular metabolic needs during exponential growth. The expression of carbon catabolic genes increased linearly with decreasing growth rates upon limitation of carbon influx, but decreased linearly with decreasing growth rate upon limitation of nitrogen or sulphur influx. In contrast, the expression of biosynthetic genes showed the opposite linear growth-rate dependence as the catabolic genes. A coarse-grained mathematical model provides a quantitative framework for understanding and predicting gene expression responses to catabolic and anabolic limitations. A scheme of integral feedback control featuring the inhibition of cAMP signalling by metabolic precursors is proposed and validated. These results reveal a key physiological role of cAMP-dependent catabolite repression: to ensure that proteomic resources are spent on distinct metabolic sectors as needed in different nutrient environments. Our findings underscore the power of quantitative physiology in unravelling the underlying functions of complex molecular signalling networks.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000323112400025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 76; ARTICLE; 7462; 301-306; 500
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/391231]
专题	生命科学学院
推荐引用方式 GB/T 7714	You, Conghui,Okano, Hiroyuki,Hui, Sheng,et al. Coordination of bacterial proteome with metabolism by cyclic AMP signalling[J]. nature,2013.
APA	You, Conghui.,Okano, Hiroyuki.,Hui, Sheng.,Zhang, Zhongge.,Kim, Minsu.,...&Hwa, Terence.(2013).Coordination of bacterial proteome with metabolism by cyclic AMP signalling.nature.
MLA	You, Conghui,et al."Coordination of bacterial proteome with metabolism by cyclic AMP signalling".nature (2013).