Differential Sensitivity of Bat Cells to Infection by Enveloped RNA   Viruses: Coronaviruses, Paramyxoviruses, Filoviruses, and Influenza   Viruses

doi:10.1371/journal.pone.0072942

CORC > 北京大学 > 生命科学学院

	Differential Sensitivity of Bat Cells to Infection by Enveloped RNA Viruses: Coronaviruses, Paramyxoviruses, Filoviruses, and Influenza Viruses
	Hoffmann, Markus ; Mueller, Marcel Alexander ; Drexler, Jan Felix ; Glende, Joerg ; Erdt, Meike ; Guetzkow, Tim ; Losemann, Christoph ; Binger, Tabea ; Deng, Hongkui ; Schwegmann-Wessels, Christel ; Esser, Karl-Heinz ; Drosten, Christian ; Herrler, Georg
刊名	plos one
	2013
关键词	RESPIRATORY SYNDROME CORONAVIRUS SARS-LIKE CORONAVIRUS SPIKE-PROTEIN S PROTEIN BOVINE CORONAVIRUS FRUIT BATS C VIRUS RECEPTOR DETERMINANT PROTEOLYTIC CLEAVAGE FUNCTIONAL RECEPTOR
DOI	10.1371/journal.pone.0072942
英文摘要	Bats (Chiroptera) host major human pathogenic viruses including corona-, paramyxo, rhabdo- and filoviruses. We analyzed six different cell lines from either Yinpterochiroptera (including African flying foxes and a rhinolophid bat) or Yangochiroptera (genera Carollia and Tadarida) for susceptibility to infection by different enveloped RNA viruses. None of the cells were sensitive to infection by transmissible gastroenteritis virus (TGEV), a porcine coronavirus, or to infection mediated by the Spike (S) protein of SARS-coronavirus (SARS-CoV) incorporated into pseudotypes based on vesicular stomatitis virus (VSV). The resistance to infection was overcome if cells were transfected to express the respective cellular receptor, porcine aminopeptidase N for TGEV or angiotensin-converting enzyme 2 for SARS-CoV. VSV pseudotypes containing the S proteins of two bat SARS-related CoV (Bg08 and Rp3) were unable to infect any of the six tested bat cell lines. By contrast, viral pseudotypes containing the surface protein GP of Marburg virus from the family Filoviridae infected all six cell lines though at different efficiency. Notably, all cells were sensitive to infection by two paramyxoviruses (Sendai virus and bovine respiratory syncytial virus) and three influenza viruses from different subtypes. These results indicate that bat cells are more resistant to infection by coronaviruses than to infection by paramyxoviruses, filoviruses and influenza viruses. Furthermore, these results show a receptor-dependent restriction of the infection of bat cells by CoV. The implications for the isolation of coronaviruses from bats are discussed.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000323880200055&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 18; ARTICLE; 8; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/391183]
专题	生命科学学院
推荐引用方式 GB/T 7714	Hoffmann, Markus,Mueller, Marcel Alexander,Drexler, Jan Felix,et al. Differential Sensitivity of Bat Cells to Infection by Enveloped RNA Viruses: Coronaviruses, Paramyxoviruses, Filoviruses, and Influenza Viruses[J]. plos one,2013.
APA	Hoffmann, Markus.,Mueller, Marcel Alexander.,Drexler, Jan Felix.,Glende, Joerg.,Erdt, Meike.,...&Herrler, Georg.(2013).Differential Sensitivity of Bat Cells to Infection by Enveloped RNA Viruses: Coronaviruses, Paramyxoviruses, Filoviruses, and Influenza Viruses.plos one.
MLA	Hoffmann, Markus,et al."Differential Sensitivity of Bat Cells to Infection by Enveloped RNA Viruses: Coronaviruses, Paramyxoviruses, Filoviruses, and Influenza Viruses".plos one (2013).