Single-cell clones of liver cancer stem cells have the potential of   differentiating into different types of tumor cells

doi:10.1038/cddis.2013.340

CORC > 北京大学 > 生命科学学院

	Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells
	Liu, H. ; Zhang, W. ; Jia, Y. ; Yu, Q. ; Grau, G. E. ; Peng, L. ; Ran, Y. ; Yang, Z. ; Deng, H. ; Lou, J.
刊名	cell death disease
	2013
关键词	CSCs carcinoma/cancer microenvironments multilineage differentiation potential Oct4 microvascular endothelial cells OVARIAN-CANCER DEDIFFERENTIATION MICROENVIRONMENT PLURIPOTENCY CD133 OCT4 EXPRESSION CONTRIBUTE DORMANCY HYPOXIA
DOI	10.1038/cddis.2013.340
英文摘要	Cancer stem cells (CSCs) are believed to be a promising target for cancer therapy because these cells are responsible for tumor development, maintenance and chemotherapy resistance. Finding out the critical factors regulating CSC fate is the key for target therapy of CSCs. Just as normal stem cells are regulated by their microenvironment (niche), CSCs are also regulated by cells in the tumor microenvironment. However, whether various tumor microenvironments can induce CSCs to differentiate into different cancer cells is not clear. Here, we show that single-cell-cloned CSCs, accidentally obtained from a human liver cancer microvascular endothelial cells, express classic stem cell markers, genes associated with self-renewal and pluripotent factors and possess colony-forming ability in vitro and the ability of serial transplantation in vivo. The single-cell-cloned CSCs treated with the different tumor cell/tissue-derived conditioned culture medium, which is a mimic of carcinoma microenvironment, could differentiate into corresponding tumor cells and express specific markers of the respective type of tumor cells at the gene, protein and cell levels, respectively. Interestingly, this multilineage differentiation potential of single-cell-cloned liver CSCs sharply declined after the specific knockdown of octamer-binding transcription factor 4 (Oct4) alone, even though they were under the same induction conditions (carcinoma microenvironments). These data support the hypothesis that single-cell-cloned liver CSCs have the potential of differentiating into different types of tumor cells, and the tumor microenvironment does play a crucial role in deciding differentiation directions. Simultaneously, Oct4 in CSCs is indispensable in this process. These factors are promising targets for liver CSC-specific therapy.; Cell Biology; SCI(E); 1; ARTICLE; 4
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/390991]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, H.,Zhang, W.,Jia, Y.,et al. Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells[J]. cell death disease,2013.
APA	Liu, H..,Zhang, W..,Jia, Y..,Yu, Q..,Grau, G. E..,...&Lou, J..(2013).Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells.cell death disease.
MLA	Liu, H.,et al."Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells".cell death disease (2013).