Evidence that HetR protein is an unusual serine-type protease

doi:10.1073/pnas.95.9.4959

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	Evidence that HetR protein is an unusual serine-type protease
	Zhou, RB ; Wei, XC ; Jiang, N ; Li, HG ; Dong, YQ ; Hsi, KL ; Zhao, JD
刊名	proceedings of the national academy of sciences of the united states of america
	1998
关键词	cyanobacteria Anabaena PCC7120 heterocyst differentiation HETEROCYST DIFFERENTIATION POLYVINYLIDENE DIFLUORIDE PROTEOLYSIS ANABAENA GENE SITE EXPRESSION MEMBRANES SEQUENCE
DOI	10.1073/pnas.95.9.4959
英文摘要	The hetR gene plays a very important role in cell differentiation of heterocystous cyanobacteria, To understand the mechanism of the hetR gene product in regulation of heterocyst differentiation, the recombinant HetR protein (rHetR) was overproduced in Escherichia coli, Purified rHetR was unstable and degraded easily in solution, Phenylmethanesulfonyl fluoride, a serine-type protease inhibitor, prevented the degradation and was shown to modify covalently rHetR, Dansyl fluoride (DnsF), another serine-type protease inhibitor, also covalently modifies rHetR as shown by electrophoresis and electroblotting of the labeled rHetR and by MS. The labeling of rHetR with phenylmethanesulfonyl fluoride and DnsF was at the same site of rHetR and required Ca2+. S179N-rHetR, a mutant protein from strain 216 of Anabaena PCC 7120, which cannot differentiate heterocysts because of the mutation, was also overproduced and characterized. Although S170N-rHetR still can be labeled with DnsF, no proteolysis was observed, suggesting that Ser179 is involved in proteolytic activity. DnsF-labeled rHetR was digested with trypsin, and the labeled peptide was isolated and sequenced, The labeled peptide matches a sequence from HetR, These results show that HetR is a protease.; Multidisciplinary Sciences; SCI(E); 77; 9; 4959-4963; 95
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/390214]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhou, RB,Wei, XC,Jiang, N,et al. Evidence that HetR protein is an unusual serine-type protease[J]. proceedings of the national academy of sciences of the united states of america,1998.
APA	Zhou, RB.,Wei, XC.,Jiang, N.,Li, HG.,Dong, YQ.,...&Zhao, JD.(1998).Evidence that HetR protein is an unusual serine-type protease.proceedings of the national academy of sciences of the united states of america.
MLA	Zhou, RB,et al."Evidence that HetR protein is an unusual serine-type protease".proceedings of the national academy of sciences of the united states of america (1998).