Molecular mapping of split rice spikelet mutant srs-1 and analysis of   its homeotic function in rice

doi:10.1007/BF02879301

CORC > 北京大学 > 生命科学学院

	Molecular mapping of split rice spikelet mutant srs-1 and analysis of its homeotic function in rice
	Bai, SL ; Liu, YS ; Sun, JS ; Zhu, LH
刊名	science in china series c life sciences
	2000
关键词	Oryza sativa RAPD RFLP srs-1 gene mapping homeotic gene GENE EXPRESSION PLANTS
DOI	10.1007/BF02879301
英文摘要	srs-1, a new floral organ identity gene in rice, was mapped using RAPD and RFLP markers. Firstly, the cross was made between "ZhaiYeQing 8" (ZYQ8, indica) and split rice spikelet (SRS, japonica) mutant, The ratio of wild-type individuals and mutant plants in F-2 population is 3 : 1, which indicates that the mutant characteristics are controlled by single recessive gene, srs-1. Consequently, BSA method was adopted and 520 random 10-mer primers were used to screen polymorphic bands between two bulks. Six primers could amplify polymorphic bands, of which S465 generates the most stable RAPD patterns. Then, S465 that cosegregates in F-2 population has been converted into an RFLP marker successfully. Furthermore, srs-1 gene was mapped on chromosome 3 using DH mapping population. The effect of srs-1 gene results in the mutant of split rice spikelet, The mutant has longer and softer palea/lemma than those of wild-type, and two small palea/lemma-like organs between palea and lemma. In addition, there is a flower with three stamens and carpel in the axil of lemma. Thus, there are nine stamens and two carpels in the spikelet of mutant. srs-1 gene may belong to homeotic gene of class A according to the mutant characteristics and ABC model.; Biology; SCI(E); 2; ARTICLE; 4; 369-375; 43
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/389373]
专题	生命科学学院
推荐引用方式 GB/T 7714	Bai, SL,Liu, YS,Sun, JS,et al. Molecular mapping of split rice spikelet mutant srs-1 and analysis of its homeotic function in rice[J]. science in china series c life sciences,2000.
APA	Bai, SL,Liu, YS,Sun, JS,&Zhu, LH.(2000).Molecular mapping of split rice spikelet mutant srs-1 and analysis of its homeotic function in rice.science in china series c life sciences.
MLA	Bai, SL,et al."Molecular mapping of split rice spikelet mutant srs-1 and analysis of its homeotic function in rice".science in china series c life sciences (2000).