Sequence-specific assignment of H-1-NMR resonance and determination of the secondary structure of HWTX-II | |
Shu, Q ; Lu, SY ; Gu, XC ; Liang, SP | |
刊名 | acta biochimica et biophysica sinica
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2001 | |
关键词 | HWTX-II 2D-NMR sequence-specific assignment secondary structure SPIDER SELENOCOSMIA-HUWENA CALCIUM-CHANNEL ANTAGONIST NUCLEAR-MAGNETIC-RESONANCE FUNNEL-WEB SPIDER 3-DIMENSIONAL STRUCTURE CYSTINE KNOT HUWENTOXIN-I VENOM POLYPEPTIDES NEUROTOXINS |
英文摘要 | Huwencoxin-II (HWTX-II) is an insecticidal peptide purified from the venom of spider Selenocosmia huwena. The structure of this toxin in solution was investigated using 2D-NMR. The complete sequence-specific assignments of proton resonance in the H-1-NMR spectra of HWTX-II were obtained by analyzing a series of 2D spectrum, including COSY, DQF-COSY, TOCSY and NOESY. All the backbone protons and side chain protons, except epsilon NH2 protons of Lys residues, were identified by d(alphaN), d(alpha delta), d(NN) and d(betaN) connectivities. The results provide a basis for further determination of the solution conformation of HWTX-II. Furthermore the secondary structure of HWTX-II was determined from NMR data. It contained mainly extended conformation, especially a double-stranded anti-parallel beta -sheet with Trp27-Cys29 and Cys34-Lys36 at the C terminal, and it lacked helix. These characters of the secondary structure of HWTX-II were similar to those spider toxins which structure in solution had been reported.; Biochemistry & Molecular Biology; Biophysics; SCI(E); 9; ARTICLE; 1; 65-70; 33 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/389334] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Shu, Q,Lu, SY,Gu, XC,et al. Sequence-specific assignment of H-1-NMR resonance and determination of the secondary structure of HWTX-II[J]. acta biochimica et biophysica sinica,2001. |
APA | Shu, Q,Lu, SY,Gu, XC,&Liang, SP.(2001).Sequence-specific assignment of H-1-NMR resonance and determination of the secondary structure of HWTX-II.acta biochimica et biophysica sinica. |
MLA | Shu, Q,et al."Sequence-specific assignment of H-1-NMR resonance and determination of the secondary structure of HWTX-II".acta biochimica et biophysica sinica (2001). |
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