Molecular cloning, sequencing, and expression in Escherichia coli of the chalcone synthase gene | |
Shao L ; Li Y ; Pan A ; Cheng Z ; Chen M | |
刊名 | 生物工程学报
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1995 | |
英文摘要 | Flavonoids form a class of secondary metabolites that are abundant in all higher plants. They serve important functions in flower pigmentation. Chalcone Synthase (CHS) is a key enzyme in the biosynthesis of all classes of flavonoids; therefore, variation of the expression of CHS might change the color of flowers. We cloned CHS-A gene from Petunia hybrida, which has 99% sequence homology with those that have been cloned abroad. The sequence of the coding region is 1170bp and encodes a protein of 39kDa. CHS-A gene is also expressed high-efficiently in E. coli. All this work establishes a sound basis for future research on the impact of the CHS-A gene on flower pigmentation.; PubMed; 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 0; 2; 131-5; 11 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/347995] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Shao L,Li Y,Pan A,et al. Molecular cloning, sequencing, and expression in Escherichia coli of the chalcone synthase gene[J]. 生物工程学报,1995. |
APA | Shao L,Li Y,Pan A,Cheng Z,&Chen M.(1995).Molecular cloning, sequencing, and expression in Escherichia coli of the chalcone synthase gene.生物工程学报. |
MLA | Shao L,et al."Molecular cloning, sequencing, and expression in Escherichia coli of the chalcone synthase gene".生物工程学报 (1995). |
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