Regulating function of enzymization and deenzymization of the lactate dehydrogenase isozymes in the mouse tissues during hypoxia

CORC > 北京大学 > 生命科学学院

	Regulating function of enzymization and deenzymization of the lactate dehydrogenase isozymes in the mouse tissues during hypoxia
	Yuan M ; Shao H ; Geng Z ; Liu J ; Deng A
刊名	zhongguo yi xue ke xue yuan xue bao acta academiae medicinae sinicae
	1998
英文摘要	Study the characteristics of changes of LDH enzyme map of mouse under slight hypoxia.Treated mouse with artificial hypoxia, various tissue biopsies were made for the test of LDH enzymatic activity by specific staining technical LDH(1-5)relative percent enzymatic activity were measured with CS-910 dual-wavelength thin-layer chromatography scanner.The relative percent enzymatic activity of LDH isozymes of various tissues after slight hypoxia shift to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and the relative percent enzymatic activity of LDH1 (H4), LDH2-(H3M) increased (the relative percent enzymatic activity of LDH2 increased markedly, P < 0.05), while LDH (M4) in various tissues decreased prominently except the cardiac muscle, and that of LDH4 (HM3) decreased as well. After the PAGE of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme pattern of LDH, with the regularity of LDH1 (0 subband), LDH2 (0-1 subbands), LDH3 (0-2 subbands), LDH4 (1-3 subbands), LDH5 (2-4 subbands). After adding appropriate amount of NAD+ to mouse cardiac muscle specimen with hypoxia, PAGE showed the numbers of subbands of four kinds of isozymes (LDH2-LDH5) decreased even totally disappeared in the isozyme pattern.The negative feedback regulation of enzymization and deenzymization of LDH isozymes is one of mouse stress responses to slight hypoxia.; PubMed; 0; 6; 449-53; 20
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/347422]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yuan M,Shao H,Geng Z,et al. Regulating function of enzymization and deenzymization of the lactate dehydrogenase isozymes in the mouse tissues during hypoxia[J]. zhongguo yi xue ke xue yuan xue bao acta academiae medicinae sinicae,1998.
APA	Yuan M,Shao H,Geng Z,Liu J,&Deng A.(1998).Regulating function of enzymization and deenzymization of the lactate dehydrogenase isozymes in the mouse tissues during hypoxia.zhongguo yi xue ke xue yuan xue bao acta academiae medicinae sinicae.
MLA	Yuan M,et al."Regulating function of enzymization and deenzymization of the lactate dehydrogenase isozymes in the mouse tissues during hypoxia".zhongguo yi xue ke xue yuan xue bao acta academiae medicinae sinicae (1998).