Tetragonal Crystal Structure of Mung Bean Inhibitor-porcine Trypsin Complex

CORC > 北京大学 > 生命科学学院

	Tetragonal Crystal Structure of Mung Bean Inhibitor-porcine Trypsin Complex
	Liu Xiao-Zhou ; Liu Bo ; Lu Guang-Ying ; Wei Xin-Cheng ; Hua Zi-Qian ; Chi Cheng-Wu
刊名	sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica
	1998
英文摘要	Crystal structure of the mung bean inhibitor-porcine trypsin (1:2) ternary complex in the tetragonal crystal with space group I422 was determined at 0.25nm resolution. 56 residues of the mung bean inhibitor were resolved among which one more residue Pro11 was determined than the previously reported structure in the trigonal crystal of the same complex. The structure of the inhibitor in the tetragonal crystal is similar to that in the trigonal crystal and the complexes in tetragonal crystal is also in packing disorder as in trigonal crystal, i.e., the complexes pack in two orientations Ta : MaMb : Tb and Tb : MbMa : Ta(Ta, Tb=trypsin, Ma, Mb=loop I and loop II of mung bean inhibitor respectively). But there are some differences in the two crystal forms. First, the inhibitor in the tetragonal crystal has no pseudo- beta-sheet structure which the trigonal crystal has. Second, its conformation is somewhat different from that in the trigonal crystal.Analysis showed that the linkage peptides between the two regid domains of the inhibitor were flexible, which also accounted for the formation of different crystal forms of this complex. Moreover, comparing mung bean inhibitor to other Bowman-Birk inhibitors showed that the two double-stranded antiparallel beta-sheets and the reactive binding loops were highly conservative.; PubMed; 0; 4; 357-362; 30
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/347304]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu Xiao-Zhou,Liu Bo,Lu Guang-Ying,et al. Tetragonal Crystal Structure of Mung Bean Inhibitor-porcine Trypsin Complex[J]. sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica,1998.
APA	Liu Xiao-Zhou,Liu Bo,Lu Guang-Ying,Wei Xin-Cheng,Hua Zi-Qian,&Chi Cheng-Wu.(1998).Tetragonal Crystal Structure of Mung Bean Inhibitor-porcine Trypsin Complex.sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica.
MLA	Liu Xiao-Zhou,et al."Tetragonal Crystal Structure of Mung Bean Inhibitor-porcine Trypsin Complex".sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica (1998).