Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering

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	Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering
	Wang Dayong ; Xu Yunjian ; Li Qing ; Hao Xuemei ; Cui Keming ; Sun Fangzhen ; Zhu Yuxian
刊名	the plant journal for cell and molecular biology
	2003
英文摘要	PPF1 is a gibberellin-induced, vegetative growth-specific gene, first isolated from short-day (SD)-grown G2 pea plants. In the current work, we found that transgenic Arabidopsis plants overexpressing the PPF1 gene (PPF1 (+)) flowered much later and had a significantly longer lifespan compared to control plants, whereas suppression of this gene (PPF1 (-)) resulted in a very rapid reproductive cycle. Western blotting analyses of PPF1 (+) and (-) plant lines revealed a positive correlation between the amount of antibody-reactive protein and the time of flowering. Green fluorescent protein (GFP) co-expression assays showed that the PPF1 protein is likely localized in chloroplast membranes. Transgenic expression of PPF1 affected the calcium storage capacities since chloroplasts isolated from PPF1 (+) plants contained high Ca2+ levels while chloroplasts of PPF1 (-) plants contained very low amounts of calcium ion. Using Novikoff human hepatoma cells, we demonstrated that expression of PPF1 leads to a significant inward calcium ion current that was absent in untransformed cells. We conclude that, as a putative calcium ion carrier, PPF1 affects the flowering time of higher plants by modulating Ca2+ storage capacity within chloroplasts.; SCI(E); EI; PubMed; 0; 2; 285-92; 33
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/347236]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang Dayong,Xu Yunjian,Li Qing,et al. Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering[J]. the plant journal for cell and molecular biology,2003.
APA	Wang Dayong.,Xu Yunjian.,Li Qing.,Hao Xuemei.,Cui Keming.,...&Zhu Yuxian.(2003).Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering.the plant journal for cell and molecular biology.
MLA	Wang Dayong,et al."Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering".the plant journal for cell and molecular biology (2003).