Genome-wide ORFeome cloning and analysis of Arabidopsis transcription   factor genes

doi:10.1104/pp.104.042176

CORC > 北京大学 > 生命科学学院

	Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes
	Gong, W ; Shen, YP ; Ma, LG ; Pan, Y ; Du, YL ; Wang, DH ; Yang, JY ; Hu, LD ; Liu, XF ; Dong, CX ; Ma, L ; Chen, YH ; Yang, XY ; Gao, Y ; Zhu, DM ; Tan, XL ; Mu, JY ; Zhang, DB ; Liu, YL ; Dinesh-Kumar, SP ; Li, Y ; Wang, XP ; Gu, HY ; Qu, LJ ; Bai, SN ; Lu, YT ; Li, JY ; Zhao, JD ; Zuo, JR ; Huang, H ; Deng, XW ; Zhu, YX
刊名	plant physiology
	2004
关键词	MADS-BOX GENE FACTOR FAMILY SEEDLING DEVELOPMENT EXPRESSION PROTEIN THALIANA APETALA1 ENCODES PISTILLATA MERISTEMS
DOI	10.1104/pp.104.042176
英文摘要	Here, we report our effort in generating an ORFeome collection for the Arabidopsis transcription factor (TF) genes. In total, ORFeome clones representing 1,282 Arabidopsis TF genes have been obtained in the Gateway high throughput cloning pENTR vector, including 411 genes whose annotation lack cDNA support. All the ORFeome inserts have also been mobilized into a yeast expression destination vector, with an estimated 85% rate of expressing the respective proteins. Sequence analysis of these clones revealed that 34 of them did not match with either the reported cDNAs or current predicted open-reading-frame sequences. Among those, novel alternative splicing of TF gene transcripts is responsible for the observed differences in at least five genes. However, those alternative splicing events do not appear to be differentially regulated among distinct Arabidopsis tissues examined. Lastly, expression of those TF genes in 17 distinct Arabidopsis organ types and the cultured cells was profiled using a 70-mer oligo microarray.; Plant Sciences; SCI(E); EI; PubMed; 0; ARTICLE; 2; 773-782; 135
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/346889]
专题	生命科学学院
推荐引用方式 GB/T 7714	Gong, W,Shen, YP,Ma, LG,et al. Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes[J]. plant physiology,2004.
APA	Gong, W.,Shen, YP.,Ma, LG.,Pan, Y.,Du, YL.,...&Zhu, YX.(2004).Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes.plant physiology.
MLA	Gong, W,et al."Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes".plant physiology (2004).