Ca2+-induced Ca2+ release in sensory neurons - Low gain amplification   confers intrinsic stability

doi:10.1074/jbc.C500026200

CORC > 北京大学 > 生命科学学院

	Ca2+-induced Ca2+ release in sensory neurons - Low gain amplification confers intrinsic stability
	Ouyang, K ; Wu, CH ; Cheng, HP
刊名	journal of biological chemistry
	2005
关键词	HEART-MUSCLE SARCOPLASMIC-RETICULUM RYANODINE RECEPTORS CALCIUM-RELEASE LOCAL-CONTROL CARDIAC MYOCYTES SKELETAL-MUSCLE CELLS SPARKS ADAPTATION
DOI	10.1074/jbc.C500026200
英文摘要	Ca2+-induced Ca2+ release (CICR) is a ubiquitous mechanism by which Ca2+ release from the endoplasmic reticulum amplifies the trigger Ca2+ entry and generates propagating Ca2+ waves. To elucidate the mechanisms that control this positive feedback, we investigated the spatial and temporal kinetics and measured the gain function of CICR in small sensory neurons from mammalian dorsal root ganglions ( DRGs). We found that subsurface Ca2+ release units (CRUs) are under tight local control by Ca2+ entry, whereas medullar CRUs as a "common pool" system are recruited by inwardly propagating CICR. Active CRUs often displayed repetitive Ca2+ sparks, conferring the ability to encode a "memory" of neuronal activity well beyond the duration of an action potential. Store Ca2+ reserve was able to support all CRUs each to fire similar to 15 sparks, excluding use-dependent inactivation or store depletion as the major CICR termination mechanism. Importantly, CICR in DRG neurons operated in a low gain, linear regime ( gain = 0.54), which conferred intrinsic stability to CICR. Combined with high Ca2+ current density ( - 156 pA/pF at - 10 mV), such a low gain CICR system generated large intracellular Ca2+ transients without jeopardizing the stability. These findings provide the first demonstration that CICR operating in a low gain regime can be harnessed to provide a robust and graded amplification of Ca2+ signal in the absence of counteracting inhibitory mechanism.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000228444800055&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); EI; PubMed; 15; ARTICLE; 16; 15898-15902; 280
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/346425]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ouyang, K,Wu, CH,Cheng, HP. Ca2+-induced Ca2+ release in sensory neurons - Low gain amplification confers intrinsic stability[J]. journal of biological chemistry,2005.
APA	Ouyang, K,Wu, CH,&Cheng, HP.(2005).Ca2+-induced Ca2+ release in sensory neurons - Low gain amplification confers intrinsic stability.journal of biological chemistry.
MLA	Ouyang, K,et al."Ca2+-induced Ca2+ release in sensory neurons - Low gain amplification confers intrinsic stability".journal of biological chemistry (2005).