Identification of an antigenic determinant on the S2 domain of the   severe acute respiratory syndrome coronavirus spike glycoprotein capable   of inducing neutralizing antibodies

doi:10.1128/JVI.78.13.6938-6945.2004

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	Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies
	Zhang, H ; Wang, GW ; Li, H ; Nie, YC ; Shi, XL ; Lian, GW ; Wang, W ; Yin, XL ; Zhao, Y ; Qu, XX ; Ding, MX ; Deng, HK
刊名	病毒学杂志
	2004
关键词	INFECTIOUS-BRONCHITIS VIRUS MURINE CORONAVIRUS SARS CORONAVIRUS PROTEOLYTIC CLEAVAGE PEPLOMER GLYCOPROTEIN FUNCTIONAL RECEPTOR GENOME EXPRESSION AMINO-ACIDS CELL-FUSION STRAIN-JHM
DOI	10.1128/JVI.78.13.6938-6945.2004
英文摘要	Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a newly identified coronavirus (CoV), SARS-CoV. The spike (S) glycoprotein of CoV is the major structural protein responsible for induction of host immune response and virus neutralization by antibodies. Hence, knowledge of neutralization determinants on the S protein is helpful for designing protective vaccines. To analyze the antigenic structure of the SARS-CoV S2 domain, the carboxyl-terminal half of the S protein, we first used sera from convalescent SARS patients to test the antigenicity of 12 overlapping fragments spanning the entire S2 and identified two antigenic determinants (Leu 803 to Ala 828 and Pro 1061 to Ser 1093). To determine whether neutralizing antibodies can be elicited by these two determinants, we immunized animals and found that both of them could induce the S2-specific antisera. In some animals, however, only one determinant (Leu 803 to Ala 828) was able to induce the antisera with the binding ability to the native S protein and the neutralizing activity to the SARS-CoV pseudovirus. This determinant is highly conserved across different SARS-CoV isolates. Identification of a conserved antigenic determinant on the S2 domain of the SARS-CoV S protein, which has the potential for inducing neutralizing antibodies, has implications in the development of effective vaccines against SARS-CoV.; Virology; SCI(E); PubMed; 65; ARTICLE; 13; 6938-6945; 78
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/346224]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, H,Wang, GW,Li, H,et al. Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies[J]. 病毒学杂志,2004.
APA	Zhang, H.,Wang, GW.,Li, H.,Nie, YC.,Shi, XL.,...&Deng, HK.(2004).Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies.病毒学杂志.
MLA	Zhang, H,et al."Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies".病毒学杂志 (2004).