Molecular cloning, phylogenetic analysis, expressional profiling and in   vitro studies of TINY2 from Arabidopsis thaliana

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	Molecular cloning, phylogenetic analysis, expressional profiling and in vitro studies of TINY2 from Arabidopsis thaliana
	Wei, G ; Pan, Y ; Lei, J ; Zhu, YX
刊名	journal of biochemistry and molecular biology
	2005
关键词	ABA DREB one-hybrid stress transcription factor LOW-TEMPERATURE GENE-EXPRESSION TRANSCRIPTION FACTORS AP2 DOMAIN DEHYDRATION DROUGHT STRESS ABA APETALA2 ELEMENT
英文摘要	A cDNA that was rapidly induced upon abscisic acid, cold, drought, mechanical wounding and to a lesser extent, by high salinity treatment, was isolated from Arabidopsis seedlings. It was classified as DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and was closely related to the TINY gene, we named it TINY2. Gel retardation assay revealed that TINY2 was able to form a specific complex with the previously characterized DRE element while showed only residual affinity to the GCC box. When fused to the GAL4 DNA-binding domain, either full-length or its C-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay while its N-terminus was completely inactive. Our data indicate that TINY2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream genes in response to environmental stress.; Biochemistry & Molecular Biology; SCI(E); PubMed; 26; ARTICLE; 4; 440-446; 38
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/346218]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wei, G,Pan, Y,Lei, J,et al. Molecular cloning, phylogenetic analysis, expressional profiling and in vitro studies of TINY2 from Arabidopsis thaliana[J]. journal of biochemistry and molecular biology,2005.
APA	Wei, G,Pan, Y,Lei, J,&Zhu, YX.(2005).Molecular cloning, phylogenetic analysis, expressional profiling and in vitro studies of TINY2 from Arabidopsis thaliana.journal of biochemistry and molecular biology.
MLA	Wei, G,et al."Molecular cloning, phylogenetic analysis, expressional profiling and in vitro studies of TINY2 from Arabidopsis thaliana".journal of biochemistry and molecular biology (2005).