Cloning and functional characterization of two cDNAs encoding   NADPH-dependent 3-ketoacyl-CoA reductased from developing cotton fibers

doi:10.1038/sj.cr.7290315

CORC > 北京大学 > 生命科学学院

	Cloning and functional characterization of two cDNAs encoding NADPH-dependent 3-ketoacyl-CoA reductased from developing cotton fibers
	Qin, YM ; Pujol, FMA ; Shi, YH ; Feng, JX ; Liu, YM ; Kastaniotis, AJ ; Hiltunen, JK ; Zhu, YX
刊名	细胞研究英文版
	2005
关键词	very-long-chain fatty acids endoplasmic reticulum fatty acid elongation system 3-ketoacyl-CoA reductase Gossypium hirsutum short-chain alcohol dehydrogenase/reductase protein family CHAIN ELONGATION SYSTEM FATTY-ACID ELONGASE SACCHAROMYCES-CEREVISIAE ENDOPLASMIC-RETICULUM CUTICULAR WAXES GENE PROTEIN IDENTIFICATION BIOSYNTHESIS SYNTHASE
DOI	10.1038/sj.cr.7290315
英文摘要	Genes encoding enzymes involved in biosynthesis of very long chain fatty acids were significantly up-regulated during early cotton fiber development. Two cDNAs, GhKCR1 and GhKCR2 encoding putative cotton 3-ketoacyl-CoA reductases that catalyze the second step in fatty acid elongation, were isolated from developing cotton fibers. GhKCR1 and 2 contain open reading frames of 963 by and 924 by encoding proteins of 320 and 307 amino acid residues, respectively. Quantatitive RT-PCR analysis showed that both these genes were highly preferentially expressed during the cotton fiber elongation period with much lower levels recovered from roots, stems and leaves. GhKCR1 and 2 showed 30%-32% identity to Saccharomyces cerevisiae Ybr159p at the deduced amino acid level. These cotton cDNAs were cloned and expressed in yeast haploid ybr159w Delta mutant that was deficient in 3-ketoacyl-CoA reductase activity. Wild-type growth rate was restored in ybr159w Delta cells that expressed either GhKCR1 or 2. Further analysis showed that GhKCR1 and 2 were co-sedimented within the membranous pellet fraction after high-speed centrifugation, similar to the yeast endoplasmic reticulum marker ScKar2p. Both GhKCR(s) showed NADPH-dependent 3-ketoacyl-CoA reductase activity in an in vitro assay system using palmitoyl-CoA and malonyl-CoA as substrates. Our results suggest that GhKCR1 and 2 are functional orthologues of ScYbr159p.; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 21; ARTICLE; 6; 465-473; 15
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/346073]
专题	生命科学学院
推荐引用方式 GB/T 7714	Qin, YM,Pujol, FMA,Shi, YH,et al. Cloning and functional characterization of two cDNAs encoding NADPH-dependent 3-ketoacyl-CoA reductased from developing cotton fibers[J]. 细胞研究英文版,2005.
APA	Qin, YM.,Pujol, FMA.,Shi, YH.,Feng, JX.,Liu, YM.,...&Zhu, YX.(2005).Cloning and functional characterization of two cDNAs encoding NADPH-dependent 3-ketoacyl-CoA reductased from developing cotton fibers.细胞研究英文版.
MLA	Qin, YM,et al."Cloning and functional characterization of two cDNAs encoding NADPH-dependent 3-ketoacyl-CoA reductased from developing cotton fibers".细胞研究英文版 (2005).