Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water

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	Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water
	Chu Ming-ming ; Guo Zhen-quan ; Muto Norio ; Itoh Norio ; Tanaka Keiichi ; Ren Hong-wei
刊名	frontiers in bioscience a journal and virtual library
	2006
英文摘要	Metallothionein (MT), a metal-binding protein induced primarily by heavy metals in vertebrates, is considered a biomarker for environmental heavy-metal contamination. To investigate heavy metal pollution in the freshwater environment, MT-I and MT-II were purified from livers of crucian carp (Carassius carassius) by gel exclusion chromatography and ion exchange chromatography. To detect the purified MT-II, a specific monoclonal antibody (mAb) against crucian carp MT-II was produced from the hybridoma strains by cell-cell fusion. By using Enzyme-Linked Immunosorbent Assay (ELISA) with this mAb, the purified crucian carp MT-II was detected with a high specificity and sensitivity. There was a good correlation between the amount of MT-II in carp livers and the concentration of heavy metals in water. ELISA was then used to evaluated the degree of heavy metal pollution in two freshwater systems. The results indicate that the MT-II content in carp liver tissue can be used as an indicator of environmental heavy-metal pollution.; SCI(E); PubMed; 0; ARTICLE; 2113-22; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345819]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chu Ming-ming,Guo Zhen-quan,Muto Norio,et al. Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water[J]. frontiers in bioscience a journal and virtual library,2006.
APA	Chu Ming-ming,Guo Zhen-quan,Muto Norio,Itoh Norio,Tanaka Keiichi,&Ren Hong-wei.(2006).Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water.frontiers in bioscience a journal and virtual library.
MLA	Chu Ming-ming,et al."Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water".frontiers in bioscience a journal and virtual library (2006).