Regulation of apoptosis and differentiation by p53 in human embryonic   stem cells

doi:10.1074/jbc.M610464200

CORC > 北京大学 > 生命科学学院

	Regulation of apoptosis and differentiation by p53 in human embryonic stem cells
	Qin, Han ; Yu, Tianxin ; Qing, Tingting ; Liu, Yanxia ; Zhao, Yang ; Cai, Jun ; Li, Jian ; Song, Zhihua ; Qu, Xiuxia ; Zhou, Peng ; Wu, Jiong ; Ding, Mingxiao ; Deng, Hongkui
刊名	journal of biological chemistry
	2007
关键词	DNA-DAMAGE SELF-RENEWAL MUTATION FREQUENCY HUMAN BLASTOCYSTS RNA INTERFERENCE PROTECTS MICE ACTIVIN-A ES CELLS EXPRESSION NANOG
DOI	10.1074/jbc.M610464200
英文摘要	The essentially infinite expansion potential and pluripotency of human embryonic stem cells (hESCs) makes them attractive for cell-based therapeutics. In contrast to mouse embryonic stem cells (mESCs), hESCs normally undergo high rates of spontaneous apoptosis and differentiation, making them difficult to maintain in culture. Here we demonstrate that p53 protein accumulates in apoptotic hESCs induced by agents that damage DNA. However, despite the accumulation of p53, it nevertheless fails to activate the transcription of its target genes. This inability of p53 to activate its target genes has not been observed in other cell types, including mESCs. We further demonstrate that p53 induces apoptosis of hESCs through a mitochondrial pathway. Reducing p53 expression in hESCs in turn reduces both DNA damage-induced apoptosis as well as spontaneous apoptosis. Reducing p53 expression also reduces spontaneous differentiation and slows the differentiation rate of hESCs. Our studies reveal the important roles of p53 as a critical mediator of human embryonic stem cells survival and differentiation.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000244482300080&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); EI; PubMed; 124; ARTICLE; 8; 5842-5852; 282
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345658]
专题	生命科学学院
推荐引用方式 GB/T 7714	Qin, Han,Yu, Tianxin,Qing, Tingting,et al. Regulation of apoptosis and differentiation by p53 in human embryonic stem cells[J]. journal of biological chemistry,2007.
APA	Qin, Han.,Yu, Tianxin.,Qing, Tingting.,Liu, Yanxia.,Zhao, Yang.,...&Deng, Hongkui.(2007).Regulation of apoptosis and differentiation by p53 in human embryonic stem cells.journal of biological chemistry.
MLA	Qin, Han,et al."Regulation of apoptosis and differentiation by p53 in human embryonic stem cells".journal of biological chemistry (2007).