Multilineage potential of pulp stem cells from human young permanent teeth in vitro | |
Liu Hong-sheng ; Bai Xiao-wen ; Yang Yuan ; Ge Li-hong | |
刊名 | 北京大学学报 医学版
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2007 | |
英文摘要 | To isolate and culture the pulp cells from human young permanent teeth (pDPC), and to observe their biological characteristics and the expression of some specific markers, and to induce these pulp cells to differentiate into osteoblast, adipocyte, neuron and chondrocyte lineages.Pulp cells were isolated and cultured from orthodontic extracted premolars of children. The attached cells after at least 3 passages were used for the following experiments: 1. Morphology and ultrastructure analysis; 2. Cell cycle and phenotype were analyzed by flowcytometry; 3. Growth curve were recorded; 4. pDPC were induced to differentiate into osteoblast, adipocyte, neuron in vitro, and were identified by histochemical methods and RT-PCR.1. Attached pDPCs were fibroblast-like cells, which were distinguished from BMSC. 2. The cell organs in dDPCs were well developed. 3. pDPCs were highly positive for CD90, CD44, CD147, which are mesenchymal stem-cell markers, but were negative for other markers including CD34, CD38, CD45, HLA-DR. 4. pDPCs showed high growth rate. 5. pDPCs could be induced to differentiate into osteoblast, adipocyte, and neuron lineages, but not chondrocyte lineages.pDPCs were characterized by their ability to proliferate with high growth rate in vitro. The expression of some BMSC markers in these cells were observed. They showed the potential to differentiate into multiple mesenchymal lineages such as osteoblast, adipocyte, neuron lineages under specific conditions in vitro.; PubMed; 0; 1; 41-5; 39 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/345484] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Liu Hong-sheng,Bai Xiao-wen,Yang Yuan,et al. Multilineage potential of pulp stem cells from human young permanent teeth in vitro[J]. 北京大学学报 医学版,2007. |
APA | Liu Hong-sheng,Bai Xiao-wen,Yang Yuan,&Ge Li-hong.(2007).Multilineage potential of pulp stem cells from human young permanent teeth in vitro.北京大学学报 医学版. |
MLA | Liu Hong-sheng,et al."Multilineage potential of pulp stem cells from human young permanent teeth in vitro".北京大学学报 医学版 (2007). |
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