Multilineage potential of pulp stem cells from human young permanent teeth in vitro

CORC > 北京大学 > 生命科学学院

	Multilineage potential of pulp stem cells from human young permanent teeth in vitro
	Liu Hong-sheng ; Bai Xiao-wen ; Yang Yuan ; Ge Li-hong
刊名	北京大学学报医学版
	2007
英文摘要	To isolate and culture the pulp cells from human young permanent teeth (pDPC), and to observe their biological characteristics and the expression of some specific markers, and to induce these pulp cells to differentiate into osteoblast, adipocyte, neuron and chondrocyte lineages.Pulp cells were isolated and cultured from orthodontic extracted premolars of children. The attached cells after at least 3 passages were used for the following experiments: 1. Morphology and ultrastructure analysis; 2. Cell cycle and phenotype were analyzed by flowcytometry; 3. Growth curve were recorded; 4. pDPC were induced to differentiate into osteoblast, adipocyte, neuron in vitro, and were identified by histochemical methods and RT-PCR.1. Attached pDPCs were fibroblast-like cells, which were distinguished from BMSC. 2. The cell organs in dDPCs were well developed. 3. pDPCs were highly positive for CD90, CD44, CD147, which are mesenchymal stem-cell markers, but were negative for other markers including CD34, CD38, CD45, HLA-DR. 4. pDPCs showed high growth rate. 5. pDPCs could be induced to differentiate into osteoblast, adipocyte, and neuron lineages, but not chondrocyte lineages.pDPCs were characterized by their ability to proliferate with high growth rate in vitro. The expression of some BMSC markers in these cells were observed. They showed the potential to differentiate into multiple mesenchymal lineages such as osteoblast, adipocyte, neuron lineages under specific conditions in vitro.; PubMed; 0; 1; 41-5; 39
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345484]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu Hong-sheng,Bai Xiao-wen,Yang Yuan,et al. Multilineage potential of pulp stem cells from human young permanent teeth in vitro[J]. 北京大学学报医学版,2007.
APA	Liu Hong-sheng,Bai Xiao-wen,Yang Yuan,&Ge Li-hong.(2007).Multilineage potential of pulp stem cells from human young permanent teeth in vitro.北京大学学报医学版.
MLA	Liu Hong-sheng,et al."Multilineage potential of pulp stem cells from human young permanent teeth in vitro".北京大学学报医学版 (2007).